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ATRA诱导下MSCs在损伤肺组织的招募及对MMP-9 mRNA的影响
Effect of ATRA on Recruiting of MSCs and MMP-9 mRNA Expression in Damage Tissue
【作者】 杨威；
【作者基本信息】 重庆医科大学 ， 外科学， 2010， 硕士
【摘要】 目的:对全反式维甲酸(All-trans retinoic acid,ATRA)诱导骨髓间充质干细胞(Mesenchymal stem cells,MSCs)在损伤肺组织的招募情况同组织中基质金属蛋白酶9信使核糖核酸(Matrix metalloproteinase 9 mRNA,MMP-9 mRNA)的表达情况进行相关性的研究。方法:对实验动物随机给予MSCs及ATRA的方式,分别于2周,3周,4周后取肺组织切片,激光共聚焦显微镜下观察植入细胞的招募情况,RT-PCR法检测组织中MMP-9 mRNA的表达变化。结果:随着时间的延长,在各时间点:在实验组,可见较多经过标记后的MSCs分布于肺泡表面,与MSCs移植组,ATRA干预组及病例对照组相比较其积分光密度(Integral optical density,IOD)明显增强,差异有统计学意义(P<0.05)。同时MMP-9 mRNA在病例对照组及ATRA干预组较高水平表达,而MSCs移植组中随着MSCs移植后时间延长,其表达水平随之降低,比较差异有统计学意义(P<0.05),而实验组MMP-9 mRNA在2周,3周,4周表达又较MSCs移植组低,两者比较差异有统计学意义(P<0.05)。结论:ATRA对肺损伤组织中移植MSCs的招募作用的影响与组织中MMP-9 mRNA水平的降低有关。
【Abstract】 Objective:To research the correlation between the effect of all-trans retinoic acid(ATRA) on recruiting MSCs and MMP-9 mRNA expression in damage tissue.Method:MSCs and ATRA were separately used in experimental SD rats.At 2 weeks,3 weeks and 4 weeks after the treatment,RT-PCR assay was used to determine expressions of MMP-9 mRNA in lung tissues.Laser scanning microscope was used to observe the recruitment of implanted MSCs.Result:We found that,at each time point,experimental group had more implanted MSCs in the surface of alveoli and increased IOD than other groups.There was significant difference in these groups(P<0.05).At the same time,in control group and ATRA intervention group,there were overexpression of MMP-9 mRNA,but in MSCs implanted group the expression of MMP-9 mRNA decreased slowly,as compared with the above two groups(P<0.05).In experimental group,the drop of MMP-9 mRNA expression was clear,as compared with the above three groups(P<0.05).Conclusion:The effect of ATRA on recruitiment of MSCs had relationship with the drop of MMP-9 mRNA expression in damage tissue.