感染白色念珠菌小鼠肾指数与病情相关性及其在中药研究中的应用

【作者】 马贤德；

【导师】 关洪全；

【作者基本信息】 辽宁中医药大学 ， 中西医结合基础， 2010， 硕士

【摘要】 目的探索感染白色念珠菌(下简称白念菌)小鼠的肾指数与其病情的相关性,用肾指数法评价小鼠感染白念菌疾病模型和相关药物对该疾病的疗效,建立一套更为简便、客观的模型评价指标。材料与方法第一部分:昆明种小鼠120只,体重20±2g,雌雄各半,随机分为4组,每组30只。分别为高剂量白念菌感染组(A组),中剂量白念菌感染组(B组),低剂量白念菌感染组(C组),空白对照组(D组)。将各组小鼠适应性饲养一周后,实验第一天,除空白对照组外,其余各组分别经尾静脉注射相应浓度的白念菌菌液,A组注射菌液浓度为4×106 CFU/ml,B组注射浓度为2×106 CFU/ml,C组为注射浓度为1×106 CFU/ml。按照0.1ml/10g体重的剂量进行注射。感染白念菌后正常饲养,实验第五天开始,隔日从各组中随机抽取5只小鼠(即:实验第5天、7天、9天、11天、13天、15天),用于检测各项指标,包括肾指数、血液载菌量、肾脏载菌量、肾脏病理(HE染色和PAS染色)、体重等。第二部分:昆明种小鼠120只,体重20±2g,雌雄各半,随机分为4组,每组30只。分别为白念菌感染组(A组),白念菌加大蒜素治疗组(B组),白念菌加氟康唑治疗组(C组),空白对照组(D组)。各组小鼠适应性饲养一周后,实验第一天,除空白对照组外,其余各组分别经尾静脉注射白念菌菌液,菌液浓度为2×106 CFU/ml,按0.1ml/10g体重的剂量进行注射。感染白念菌后正常饲养,实验第二天,B、C组分别经口灌饲相应药物予以治疗,每日一次,直至实验结束。实验第五天开始,隔日从各组中随机抽取5只小鼠(即:实验第5天、7天、9天、11天、13天、15天),用于检测各项指标,包括肾指数、血液载菌量、肾脏载菌量、肾脏病理(HE染色和PAS染色)、体重等。结果1.与空白对照组比较,高、中、低剂量白念菌感染组的肾指数均明显增大(p<0.01);大蒜素治疗组和氟康唑治疗组的肾指数均明显低于白念菌感染对照组(p<0.01)。2.肾脏载菌量的变化趋势与肾指数基本相同。与空白对照组比较,高、中、低剂量白念菌感染组的肾脏载菌量均明显增大(p<0.01);大蒜素治疗组和氟康唑治疗组的肾脏载菌量明显低于白念菌感染对照组(p<0.01)3.各组间血液载菌量的差异不明显,无明显差异(p>0.05)结论:1.感染白念菌小鼠的肾指数变化与其肾脏载菌量、病理变化呈正相关。2.肾指数能够准确的反应机体感染白念菌的程度,将肾指数纳入深部白念菌感染疾病模型的评价标准中,评价标准更具可操作性,与其他指标相比,肾指数更为简便、经济、快捷。3.肾指数可作为评价药物对白念菌感染疾病疗效的指标,用肾指数法来评价药物疗效,操作简单,经济,灵敏度高,可重复性好。更多还原

【Abstract】 ObjectiveTo explore the relationship between the weight index of kidneys and prognosis in the mice with Candida albicans infection. The renal index was used to evaluate the mice model with Candida albicans infection and the treatment efficacy with some related medicine. An convenient and objective Indicator system for evaluation of the model was established.Materials and methodsPart1:120 Kunming mice, weighing 20±2g, half male and half female, were randomly divided into four groups:Candida albicans of high-dose group (A group), Candida albicans of medium-dose group(B group), Candida albicans of low-dose group (C group), and control group (D group),each group was 30 mice. The mice of each group were raised adaptively a week, then except the control group, the mice of each group were injected the corresponding amount of Candida bacilli via the tail vein: The Candida bacilli concentration of A group was 4×106CFU/ml, B group was 2×106 CFU/ml, C group was 1×106 CFU/ml, the amount of Candida bacilli was 0.1ml/10g. The mice of each group were fed normally after infected Candida albicans.From the fifth day after infection, five mice were randomly selected from each group every other day (ie: From the first 5 days, 7 days, 9 days, 11 days, 13 days, 15 days), then the renal index, blood, Bacterial load in blood and kidney, kidney pathology (HE staining and PAS staining), body weight and so on were detected.Part2:120 Kunming mice, weighing 20±2g, half male and half female, were randomly divided into four groups:Candida albicans group (A group), Candida albicans plus allicin treatment group (B group) , Candida albicans plus fluconazole treatment group (C group), and control group (D group),each group was 30 mice. The mice of each group were raised adaptively a week, then except the control group, the mice of each group were injected Candida bacilli via the tail vein: The Candida bacilli concentration was 2×106 CFU/ml, the amount of Candida bacilli was 0.1ml/10g. The mice of each group were fed normally after infected Candida albicans, the next day after infection, the mice of B and C group were administrated drug respectively, once a day until the end of the experiment.From the fifth day after infection, five mice were randomly selected from each group every other day (ie: From the first 5 days, 7 days, 9 days, 11 days, 13 days, 15 days), then the renal index, blood, Bacterial load in blood and kidney, kidney pathology (HE staining and PAS staining), body weight and so on were detected.Results1. Compared with the control blank control group, the renal index of the mice of high-dose, medium-dose, low-dose Candida albicans infection group were significantly increased (p<0.01)2. The differences of bacterial load of kidney of each group were similar (p<0.01)3. Bacterial load of blood were not significant difference among all groups.Conclusion:1. Renal index of the mice with Candida albicans infection was positively correlated with the amount of bacterial load and the pathological changes in kidneys.2. Renal index can accurately reflect the body’s level of Candida albicans infection. It is more operable to consider renal index as the evaluation criteria of deep Candida albicans infection disease models. Compared with other indicators, renal index is more convenient, economical and fast.3. Renal index can be used as a indicator to evaluate the therapeutic efficacy of the treatment of Candida albicans infections. This kind of method is simple, economical, at the same time,has high sensitivity and good repeatability.更多还原