名贵中药哈蟆油的质量评价研究（Ⅱ）

【作者】 于洋洋；

【导师】 姜大成；

【作者基本信息】 长春中医药大学 ， 生药学， 2010， 硕士

【摘要】 目的:本课题是在前期对哈蟆油比较充分的质量评价研究基础上,转换新的角度,通过测定中国林蛙及其药用部位中重金属的含量,探讨中国林蛙生活环境中重金属与中国林蛙体内重金属含量的关联性;通过研究贮藏年限对哈蟆油主要成分的影响,找出不同贮藏年限的哈蟆油颜色深浅不同的原因;并且对哈蟆油商品进行重金属检查和微生物限度检查,研究哈蟆油药材商品的安全性。从而进一步对哈蟆油的质量问题进行评价。方法:通过测定中国林蛙雄蛙、雌蛙、哈蟆油和林蛙卵中重金属含量,并同时测定中国林蛙生活的土壤环境和水体环境中重金属含量,将其进行比对研究,同时对市场上销售的哈蟆油药材商品进行了重金属含量的测定和微生物限量检查。同时分别通过含量测定、电泳法等手段对哈蟆油储藏年限对哈蟆油的影响进行了研究。结果:实验结果显示:①中国林蛙及其药用部位的砷含量超出限量的情况比较严重,数据显示这可能是受中国林蛙生活的土壤环境中砷含量较高影响。个别样品中铜的含量有超出限量的情况。②中国林蛙生活土壤环境中镉的污染比较严重,而水体中各种重金属元素未见超出限量。③哈蟆油商品中的砷超出标准的现象比较严重。个别样品中见铜超出限量。④储藏时间长可以造成哈蟆油化学成分的变化,从而影响哈蟆油的质量。⑤目前哈蟆油药材商品染菌情况严重,这与含水量和包装方式有一定关系。结论:哈蟆油药材商品、中国林蛙及其生态环境均有不同程度的安全污染隐患,储藏时间对哈蟆油的质量有所影响。更多还原

【Abstract】 Purpose: In this study we had determined the content of heavy-metals what are in the Rana temporaria chensinensis and medicinal position,then we ratio to the content of the harmful substances in the living environment of the chinese forest frog.And we explored the relation of between the living environment security and the chinese forest frog.Method: On the one hand we had determined the content of heavy-metals in the male frog, female frog, Oviductus Ranae and frog’s egg. And we had determined the content of heavy-metals in the soil and aquatic environmental of the feed lot, and studied the association. On the other hand, we had determined the content of heavy-metals in the Oviductus Ranae what were bought from the marketplace. And we had done the microbial limit test to these wares. At the same time we had studied the effect of store-times to the Oviductus Ranae.Result: The result shows: firstly, the conten of As in chinese forest frog and the botanical source are overrun, then the date shows that may affected by the high conten of As in soil .And the conten of Cu in a part of the samples is overrun.Secondly, the content of Cd and As is high in the soil, but the content of heavy-metals is in the limit in the water. Thirdly, the conten of Cu and As is overrun in the Oviductus Ranae wares. Fourthly, the wares were infected seriously, and the results show that is relevant commodity packaging. Fifthly, the store-times can influence the quality of the Oviductus RanaeConclusion: The Oviductus Ranae wares, the chinese forest frog and the entironment have safety potential.更多还原