【作者】 李羚青；

【导师】 赵映前；

【作者基本信息】 湖北中医药大学 ， 中医临床基础， 2010， 硕士

【摘要】 1、目的运用血清药理学方法,研究牛黄参含药血清对人肝癌细胞HepG2的增殖及凋亡状况的影响,探讨其作用机制。2、方法将牛黄参胶囊原生药制成水煎剂,按一定剂量给大白兔灌胃(将大白兔分成含药血清组和空白血清组)。应用血清药理学方法,将两组血清分别稀释成不同浓度(10%、20%),并用其培养人肝癌细胞HepG2,然后使用MTT法、流式细胞术检测并比较不同浓度牛黄参(以下简称“NHS”)含药血清对肝癌细胞增殖及凋亡状况的影响。3、结果①牛黄参含药血清对肝癌细胞HepG2增殖的作用24h的10%及20%NHS含药血清的OD值与空白对照组比较有显著性差异,两组浓度的含药血清对HepG2的抑制率分别达11.53%、12.49%,20%NHS含药血清与空白血清组比较差异有统计学意义。48h的10%及20%NHS含药血清的OD值与空白对照组比较有显著差异,两组浓度的含药血清对HepG2的抑制率达16.43%及18.17%,20%NHS含药血清与空白血清组比较差异有统计学意义。②牛黄参含药血清对肝癌细胞HepG2凋亡的影响从流式细胞术检测结果来看,发现10%及20%的含药血清能促进入肝癌细胞的凋亡,与空白对照组比较有显著性差异,与空白血清组比较有显著性差异。4、结论①牛黄参含药血清能抑制肝癌细胞HepG2的增殖。②牛黄参含药血清能诱导肝癌细胞HepG2的凋亡。③牛黄参含药血清抑制肝癌细胞HepG2的增殖、诱导肝癌细胞HepG2凋亡的作用与血清浓度和培养时间相关。兔含药血清浓度越高,培养时间越长,对细胞的抑制率越高,凋亡越明显。更多还原

【Abstract】 1.PurposeThe use of serum pharmacology method to study drug-containing serum NHS Participation of human HepG2 hepatoma cell proliferation and apoptosis of the situation, and to explore its mechanism.2. MethodNHS Capsule will be made of the water decoction of native medicine, according to a certain dose to the rabbit gavage. The rabbits were divided into drug-containing serum group and blank control group, according to serum pharmacology method, serum was diluted into different concentrations (10%,20%), and with its cultured human liver cancer cell HepG2, and then use the MTT assay, flow Measuring-type cells, serum containing different concentrations of NHS on hepatoma cell proliferation and apoptosis effects.3.results①Participation NHS drug-containing serum on liver cancer cell HepG2 proliferation in vitro24h,10% and 20% NHS containing serum OD value of blank control group were significantly different, the concentration of serum containing two pairs of HepG2 inhibition rate of 11.53% and 12.49%,20% NHS containing serum and blank serum group differences were statistically significant. 48h 10% and 20% NHS containing serum OD value of blank control group were significantly different, the concentration of serum containing two pairs of HepG2 inhibition rate of 16.43% and 18.17%,20% NHS containing serum and blank serum group differences were statistically significant.②NHS Participation drug-containing serum on apoptosis of HepG2 hepatoma cellsThe results from the flow cytometry analysis point of view, found that 10% and 20% of the drug-containing serum can promote apoptosis of human hepatoma cells, and blank control group there are differences, with the blank serum group there are differences.4.Conclusion①Participation NHS containing serum can inhibit the proliferation of HepG2 hepatoma cells.②NHS containing serum parameters can induce apoptosis in HepG2 hepatoma cells.③NHS containing serum inhibited the proliferation of HepG2 hepatoma cells, induced apoptosis in HepG2 hepatoma cells and the serum concentration and culture time-dependent. Rabbit serum containing the higher the concentration, the longer the incubation time,the higher inhibition rate on cell apoptosis was more obvious.更多还原