【作者】 杨马；

【导师】 汪开毓；

【作者基本信息】 四川农业大学 ， 水产养殖， 2010， 硕士

【摘要】 本文研究了日粮中不同维生素E添加量对鲤鱼生产性能和免疫功能的影响。选择初始体重为15.11±0.10g(P>0.05)健康鲤鱼720尾,平均分成6组(分别Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ组),每组120尾,每组设三个重复,每个重复40尾,分别饲喂含维生素EO、100、500、1000、2500、5000mg.Kg-1的半纯合饵料,试验期120天.试验结果表明：维生素E对鲤鱼成活率、增重、摄饵量、饲料系数有极显著的影响(P<0.01)；同时对头肾重量、头肾体指数、脾脏重量、脾体指数、后肾重量、后肾体指数、血液白细胞吞噬活性、杀菌活性、血清溶菌酶活性、补体C。含量、抗体IgM含量、总蛋白含量、白蛋白含量、球蛋白含量以及攻毒后成活率有极显著或显著影响(P<0.01；P<0.05)。当饵料中维生素E添加量为Omg/kg时,鲤鱼成活率、增重、摄饵量极显著降低(P<0.01)；饲料系数极显著增加(P<0.01)；头肾重量、脾脏重量、血液白细胞吞噬活性、杀菌活性、血清溶菌酶活性、补体C3含量、抗体IgM含量、总蛋白含量、白蛋白含量、球蛋白含量以及攻毒后成活率极显著降低(P<0.01)；后肾重量和后肾体指数显著降低(P<0.05)；当饵料中维生素E添加量为1000mg/kg时,鲤鱼增重和摄饵量极显著增加(P<0.01)；饲料系数极显著降低(P<0.01)；头肾重量、头肾体指数、脾脏重量、脾体指数、血液白细胞吞噬活性、杀菌活性、血清溶菌酶活性、补体C3含量、抗体IgM含量以及攻毒后成活率极显著增加(P<0.01)；与需要量组相比,当饵料中E添加量为5000mg/kg时,鲤鱼增重极显著降低(P<0.01),摄饵量显著降低(P<0.05),饲料系数极显著增加(P<0.01)；头肾重量、脾脏重量极显著降低(P<0.01),头肾体指数、脾体指数、血清溶菌酶活性、抗体IgM含量、血清球蛋白含量显著降低(P<0.05),但是后肾重量、后肾体指数、血液白细胞吞噬活性、杀菌指数、血清补体C3含量、总蛋白含量、白蛋白含量没有显著变化(P>0.05)。鲤鱼增重与饲料中维生素E水平对数呈极显著的一元二次回归关系(y=-65.906+82.946x-14.949x2,R2=0.9170,P<0.01),当饵料维生素E为650.13mg/kg时增重达到最大值；头肾重量、脾脏重量、血液白细胞吞噬活性、血清溶菌酶活性、补体C3含量、抗体IgM含量、总蛋白含量与饲料中维生素E水平对数呈极显著的一元二次回归关系(R2=0.9640,P<0.01,R2=0.8880,P<0.01,R2=0.8910,P<0.01,R2=0.9730,P<0.01,R2=0.9720,P<0.01,R2=0.8470,P<0.01,R2=0.8790)；血液白细胞杀菌指数与饲料维生素E水平对数呈显著的一元二次回归关系(R2=0.6370,P<0.05)；当饵料维生素E水平为602.56mg/kg、660.70mg/kg、630.96mg/kg. 665.65mg/kg、619.44mg/kg时,鲤鱼血液白细胞吞噬活性、血清溶菌酶活性、补体C3含量、抗体IgM含量、总蛋白含量分别达到最大值。综合试验结果说明：维生素E缺乏降低鲤鱼生产性能,生产性能降低是由于鲤鱼的摄饵量降低,饲料系数提高。维生素E缺乏降低鲤鱼疾病抵抗力,疾病抵抗力下降是由于头肾和脾脏的生长受阻、免疫功能下降；在需要量基础上适当提高维生素E水平能够促进鲤鱼的生长,当维生素E水平为650.13mg/kg时,鲤鱼的生长达到最佳；在需要量基础上适当提高维生素E水平能够提高鲤鱼的疾病抵抗力,疾病抵抗力提高与维生素E促进头肾和脾脏的生长、提高非特异性和特异性免疫力有关。当维生素E水平为660.70mg/kg时,鲤鱼免疫功能最佳。更多还原

【Abstract】 The trial was conducted to study the effect of different vitamin E level in daily diet on growth performances and immune function of common carps(cyprinus carpio).In trial,720 common carps with initial 15.11±0.10g were averagely allocated to 6 treatments with 3 replications,feeding semi-purified diet supplemented with vitamin E 0,100,500,1000,2500 and 5000 mg/kg respectively for 120 days.Results indicated that:vitamin E has great significant effect on survival rate,weight gain, feed intake and feed conversion efficiency.Simultaneously,vitamin E has great significant or significant effect on head kidney weight,head kidney weight/body weight,spleen weight,spleen weight/body weight,post kidney weight,post kidney weight/body weight,phagocytic activity and bactericidal activity of phagocytes,lysozyme activity in serum,complement C3 content and IgM content in serum,total protein content in serum,albumin content and globulin content in serum,survival rate after vaccination against Aeromonas hydrophila(P<0.01)The survival rate,weight gain and feed intake decreased great significantly,feed conversion efficiency increase great significantly (P<0.01), head kidney weight,head kidney weight/body weight,spleen weight,spleen weight/body weight,phagocytic activity and bactericidal index of phagocytes,lysozyme activity in serum,complement C3 content and IgM content in serum,total protein content in serum,albumin content and globulin content in serum and survival rate after vaccination against Aeromonas hydrophila decreased great significantly(P<0.01),post kidney weight and post kidney weight/body weight decreased significantly(P<0.05) as common carps feeding diet supplemented with vitamin E 0mg/kg.The weight gain and feed intake increased great significantly,feed conversion efficiency decrease great significantly (P<0.01), head kidney weight,head kidney weight/body weight,spleen weight,spleen weight/body weight,post kidney weight and post kidney weight/body weight, phagocytic activity and bactericidal index of phagocytes,lysozyme activity in serum,complement C3 content and IgM content in serum,total protein content in serum,albumin content and globulin content in serum and survival rate after vaccination against Aeromonas hydrophila increased great significantly(P<0.01) as common carps feeding diet supplemented with vitamin E 1000mg/kg.When common carps feeding diet supplemented with vitamin E 5000mg/kg,weight gain decreased great significantly(P<0.01),feed intake decreased significantly(P<0.05),feed conversion efficiency increase great significantly (P<0.01),head kidney weight and spleen weight decrease great significantly (P<0.01),head kidney weight/body weight,spleen weight/body weight,lysozyme activity and IgM content in serum,globulin content in serum,survival rate after vaccination against Aeromonas hydrophila decreased significantly(P<0.05).But another indices,such as post kidney weight, post kidney weight/body weight, phagocytic activity and bactericidal index of phagocytes,complement C3 content in serum,total protein content and globulin content in serum were not significantly difference(P >0.05)There was significant quadraticly relationship between weight gain and lgVE (y=-65.906+82.946x-14.949x2, R2=0.9170, P<0.01).When dietary vitamin E content was 650.13mg/kg, common carps appeared the best growth performance.here was significant quadraticly relationship between weight lgVE and head kidney weight or spleen weight or phagocytic activity of phagocytes or bactericidal index of phagocytes or lysozyme activity in serum or complement C3 content in serum or IgM content in serum or total protein content in serum (R2=0.9640, P<0.01, R2=0.8880, P<0.01, R2=0.8910, P<0.01, R2=0.9730, P<0.01, R2=0.9720, P<0.01, R2=0.8470, P<0.01, R2=0.8790),When dietary vitamin E content was 602.56mg/kg,660.70mg/kg,630.96mg/kg, 665.65mg/kg,619.44mg/kg respectively,phagocytic activity of phagocytes or lysozyme activity in serum or complement C3 content in serum or IgM content in serum or total protein content in serum appeared the best value respectively.In summary,vitamin E deficiency caused growth performance of common carps decreased by decreasing feed intake and increasing feed conversion efficiency.Vitamin E deficiency caused resisting diseases ability of common carps decreased by retarding head kidney and spleen growth and decreased immune function.Increase vitamin E level in daily diet properly based on required volume could couse growth performance and immune function of common carps increased.common carps appeared best growth performance and immune function as feeding the diets containing vitamin E 650.13mg/kg and 660.70mg/kg respectively.更多还原