【作者】 吴佳理；

【导师】 张信江；

【作者基本信息】 遵义医学院 ， 皮肤病与性病学， 2010， 硕士

【摘要】 目的研究带状疱疹患者外周血单个核细胞中Toll样受体(TLR) TLR2、4、9mRNA的表达情况,并探讨其与带状疱疹的发病关系。方法采用SYBER Green I实时定量PCR方法检测30例带状疱疹患者和20例正常人的外周血单一核细胞中TLR2、TLR4、TLR9mRNA的表达水平。结果带状疱疹患者与正常人对照,其外周血单一核细胞中TLR2、TLR4、TLR9mRNA的表达水平均明显升高,其中TLR2(t=2.23,P=0.031),TLR4(t=2.32,P=0.028),TLR9(t=2.27,P=0.031),具有统计学差异(P<0.05)。结论带状疱疹患者外周血单一核细胞中TLR2、TLR4、TLR9mRNA的表达水平都明显升高,推测：在带状疱疹患者的发病过程中,TLR2、TLR4、TLR9mRNA可能参与了水痘-带状疱疹病毒的识别及抗病毒免疫。更多还原

【Abstract】 Objective To study the expression of TLR2,4,9mRNA in peripheral blood mononuclear cells (PBMC) of patients with herpes zoster, the relation between TLR and herpes zoster was discussed. Methods The expression of TLR2,4,9mRNA were detected by real-time quantitative PCR(RT-PCR) in 30 patients and 20 healthy individuals control group respectively. Rusults The experimental of TLR2,4,9mRNA were clearly higher than the healthy control group, and TLR2 (t=2.23, P=0.031), TLR4 (t=2.32, P=0.028), TLR9(t=2.27, P=0.031), the differences were statistically significant (p<0.05). Conclusion The high expression of TLR2,4,9mRNA showed that they may take part in the recognition of Varicella-zoster virus and antivirus immunity.更多还原