【作者】 寇晓文；

【导师】 熊光仲；

【作者基本信息】 中南大学 ， 急诊医学， 2010， 硕士

【摘要】 目的探讨雷帕霉素对裸鼠皮下移植胶质瘤肿瘤血管内皮生长因子(vascular endothelial growth factor VEGF)和缺氧诱导因子-1α(hypoxia inducible factor-lαHIF-α)的表达的影响及两个指标的相关性。方法建立裸鼠皮下移植胶质瘤模型,随机分组后给予：雷帕霉素常规剂量(1.5 mg／kg／d)腹腔注射,对照组给予相同体积二甲基亚砜。用药21 d后处死动物,用免疫组化法检测瘤组织中VEGF和HIF-1α表达水平。结果与对照组比较,常规剂量雷帕霉素组明显降低了肿瘤VEGF水平以及HIF-1α(P<0.05),且两个指标的表达显著相关(P<0.05)。结论常规剂量雷帕霉素可抑制肿瘤血管生成,从而抑制肿瘤生长,这为脑胶质瘤切除术后免疫抑制剂的选择提供实验依据。更多还原

【Abstract】 Objectives we approach the effect of rapamycin to the athymic mouse hypotransplantation tumor which comes from the rats’ neurospongioma.And measure the dose of VEGF (vascular endothelial growth factor, VEGF) and HIF-1α(hypoxia inducible factor-1α,HIF-1-α.).And we appraisal the effect of rapamycin to the expression of VEGFand HIF-1 aand their correlation.Methods we establish the model of the athymic mouse hypotransplantation tumor which comes from the rats neurospongioma. After randomization,we give rapamycin by mouth:the routine dose(1.5 mg/kg/d).The control group is given dimethyl sulfoxide with the same volume.Sacrifice the animals after being given the drugs, measure the dose of VEGF and HIF-1αby immunohistochemistry.Results compare to the control group,the routine dose group reduce the expression of VEGF and HIF-la(P<0.05).And the two indexs are significantly relative.Conclusions the routine dose of rapamcin can inhibit the Angiogenesis of mass,and inhibit the growth of mass,which gives the proof of choice of immunodepressant after ectomy of neurospongioma.更多还原