节点文献
慢性萎缩性胃炎热休克蛋白70表达的研究
The Studies of Expression of Heat Shock Proteins in Chronic Atrophic Gastritis and Precancerous Lesion
【作者】 吴永梅;
【导师】 谢会忠;
【作者基本信息】 新疆医科大学 , 内科学, 2009, 硕士
【摘要】 目的:探讨热休克蛋白70(heat shock proteins 70, HSP70)在人胃粘膜组织中的表达。方法:以男性161名,女性109名,共270名为研究对象,120例胃癌(在本研究中均选择的是胃窦腺癌)手术、90例慢性萎缩性胃炎伴高级别上皮内瘤变(Chronic atrophic gastritis and High intraephithelial neoplasia, CAG and H-IN)、60例慢性浅表性胃炎患者,其中胃癌患者术前均未接受放、化疗处理,经胃镜活检或术后大体标本取胃窦粘膜组织,用鼠抗HSP70的一抗、二抗及ABC试盒,工作浓度为1:400,采用免疫组化SABC方法,染色步骤按常规进行,400倍光镜观察细胞质、细胞核内棕黄色颗粒,进行阴性、阳性、中等阳性、强阳性细胞分级,以强阳性和中度阳性为过度表达。结果:1、HSP70在浅表性炎、CAG and H-IN、胃癌组织中的阳性率分别为27%、49%、67%,后二组均与浅表性胃炎组比较(P=0.000;P=0.035);过表达率分别为3%、37%、58%,后二组均与浅表性胃炎组比较(P=0.000;P=0.002),CAG and H-IN与胃窦腺癌组织比较,P>0.05;细胞核阳性过表达率0%、14%、55%,细胞浆阳性过表达率3%、7%、3%,各组细胞核阳性过表达与自身胞浆组比较,胃窦腺癌组与浅表性胃炎组(P=0.000);CAG and H-IN组(P=0.004)。2、HSP70在CAG and H-IN、胃癌组织中的表达情况与浅表性胃炎组比较有显著差异。3、HSP70在CAG and H-IN、胃癌组织中的过表达情况与浅表性胃膜组比较有显著差异;CAG and H-IN与胃窦腺癌组比较无显著差异。4、浅表性胃炎与其余各组之间HSP70过表达情况上差异较大,其余各指标之间差异不大。5、HSP70在各组细胞浆、核中的阳性过表达情况自身比较均有显著差异。结论:HSP70在胃癌和CAG and H-IN中过度表达,可能在其致病过程中起着重要的作用,进一步研究其分子机制将为胃癌的防治提供理论依据。
【Abstract】 Objective:To detere the expression of HSP70 in gastric mucosa. Methods: Immunohistochemical technique, SABC method, was used to exae the expression of HSP70 in gastric carcinoma and gastritis mucosa. Results:HSP7O was detected in 27 % of gastric epithelia,49% of dysplasia,67% of gastric carcinoma. Overexpression of HSP70 Was found in 3% of normal tissue of gastric epithelia,37% of dysplasia,58% of gastric carcinoma,0% of normal tissue of gastric epithelia,14% of dysplasia,55% of gastric carcinoma. Conclusion:The results that HSP7O protein were selectively overexpressed in gastric carcinoma suggested that HSP70 might play a specific role in the pathogenesis of gastric cancer. To clarify the mechanism of HSPs action could help to provide theorical basis for the biological therapy of gastric cancer.