【作者】 王勤；

【导师】 甘立军；

【作者基本信息】 南京农业大学 ， 植物学， 2009， 硕士

【摘要】 本论文以铜绿微囊藻(Microcystis aeruginosa)为材料,研究了不同植物生长调节剂(NAA、GA3、KT和ABA)对其生长特性的影响。研究发现不同植物生长调节剂对铜绿微囊藻的生长特性具有不同的影响：当培养液中加入生长素NAA的浓度为0.1—15 mg·L-1时,铜绿微囊藻的生物量及总的叶绿素a含量均有所增加,尤其以3mg·L-1的NAA处理效果最为明显,过高和过低浓度的NAA也促进铜绿微囊藻的生长,但效果不显著。GA3浓度在0.01—10 mg·L-范围内对铜绿微囊藻的生长和繁殖均有明显的促进作用,且随着浓度的增大作用增强,但是当浓度达到20 mg·L-1时则抑制铜绿微囊藻的生长,在开始试验的第2天就出现藻细胞黄化沉底的现象。当细胞分裂素KT的浓度在0.02—0.5mg·L-1浓度范围内时表现为延缓微囊藻细胞的生长,对照组的微囊藻细胞在培养的第7天就能进入对数生长期,而该浓度范围内处理的微囊藻细胞则在培养的第11或12天才进入对数生长期,通过测定单位干重藻细胞磷吸收量表明：在该浓度范围内,随着浓度的增加,藻细胞对磷元素的吸收量下降。当KT的处理浓度达到2.5 mg·L-1则表现为抑制铜绿微囊藻的生长,且随着浓度的增大,抑制作用愈明显,如12.5 mg·L-1和25 mg·L-1两个处理组的微囊藻细胞在培养的第2天就出现黄化沉底现象,在培养的第9天藻细胞就已全部死亡。一定浓度的ABA(0.1—1 mg·L-1)对总的叶绿素a含量、单位干重藻细胞磷吸收量和藻细胞生物量均有不同程度的促进作用,且随着浓度的增加,促进作用愈明显,但是当浓度达到5 mg·L-1则显著抑制铜绿微囊藻的生长,在开始培养的第2天,藻细胞开始出现黄化沉底的现象。试验中还对0.5 mg·L-1KT、1 mg·L-1ABA和对照组等三种处理的微囊藻细胞进行了透射电镜观察,研究发现正常铜绿微囊藻细胞一般为圆形和椭圆形,细胞内没有成形的核膜和核仁,只在细胞内存在着核区样的结构,细胞内色素以类囊体的形式附着在片层结构上,排列清晰、均匀。而0.5 mg·L-’KT处理的微囊藻细胞则表现为类囊体杂乱的分布在细胞质内,色素体含量减少,细胞核区面积减小；1mg·L-1ABA处理的微囊藻细胞则表现为色素体分布更加均匀,类囊体条数增加,所占面积增大,伸向核区,同时核区面积也增大。更多还原

【Abstract】 The effects of plant growth regulators (NAA、GA3、KT and ABA) on the growth characteristics of Microcystis aeruginosa was studied in this paper.The results indicated that different plant growth regulators had different effect on the growth of M.aeruginosa. Both the total chla contents and the cell biomass of M.aeruginosa treated with NAA (0.1—15 mg·L-1), were higher than that of the control, especialy at the concentration of 3 mg—L-1. GA3(0.01—10 mg·L-1) accelerated the total Chla contents and the cell biomass of M.aeruginosa, but higher concentrations decreased the cell biomass. For example, the cells treated by 20 mg·L-1 GA3 tended to be death at the second day of the culture time. KT(0.02—0.5mg·L-1) delaied the growth of M.aeruginosa. The cells treated with KT went in its exponential phase at the eleventh or twelvth day of the culture, which is needed only 7 days for the normal cells. It was also found that the ability of phosphorus absorption had been inhibited at these concentrations. KT at the concentration of 2.5 mg·L-1 inhibited the growth of M.aeruginosa, and the higher concentration the larger inhibition effect. KT at the concentrations of 12.5 mg·L-1 and 25 mg·L-1 decreased cell biomass and the content of Chla obviously. ABA(0.1—1 mg·L-1) accelerated the total Chla contents, the content of phosphorus absorption and the biomass, but ABA at hihger concentrations may inhibit the growth of M.aeruginosa., for example,when treated with the concentration of 5 mg·L-1, the cell tended to death at the second day of the culture time.According to the analysis of transmission electron microscopy, we found that the cell structure treated with 0.5 mg·L-1 (KT) had less thylakoid and smaller nucleus area than that of control,while the cell that treated with 1 mg·L-1 (ABA) had more thylakoid and larger nucleus area than that of the control.更多还原