【作者】 张旭；

【导师】 李任植；

【作者基本信息】 南开大学 ， 生物物理学， 2010， 硕士

【摘要】 目前电磁场刺激已被广泛应用于对骨折以及其他一些骨病的治疗,然而我们对电磁刺激作用于骨细胞的分子机制仍知之甚少。当前电磁刺激在医疗上的应用主要是根据临床经验而缺乏理论依据。因而近半个世纪以来,众多学者在该领域进行了大量的理论以及实验研究。然而,至今对于电磁场的生物学作用在科学界仍存在诸多争论。本文中,我们从理论和实验两方面对电磁刺激对于细胞胞浆钙离子以及膜电位影响进行了研究。我们采用成骨细胞作为模型来检验极低频电磁场能够改变胞浆钙离子浓度以及细胞膜电位的理论假设,并且对理论工作中所预言的窗口效应进行验证。本工作在以下两方面具有参考价值：第一,我们首次证明了极低频电磁场能够引起成骨细胞胞浆钙离子的响应,这为进一步研究电磁刺激作用于骨细胞的分子机制奠定了基础。第二,我们首次从理论和实验两方面证实了电磁刺激对于成骨细胞作用的窗口效应,并初步探寻了有效窗口,这对于电磁刺激的临床应用具有广泛的参考价值。更多还原

【Abstract】 Electromagnetic fields (EMFs) are widely used in modern medicine to heal non-unions of bone fracture and treat some bone-related diseases. Nonetheless, the specific molecular mechanisms are not fully understood. In current, the application of EMF doses on treatment is largely dependent on clinical experiences, which lacks academic supports. Thus two aspects, theoretical and empirical researches, have been emphasized in this field for almost half a century. However, still there were controversies of electromagnetic effects within the scientific community. In this work, we studied the effects of electromagnetic fields on intracellular calcium and cell membrane potential from the perspective of both theoretical and experimental works. Osteoblastic cells were used as a model both to test the hypothesis that extremely low frequency (ELF) magnetic fields can alter the concentrations of the intracellular calcium and levels of membrane potential, and to examine the’window’effect predicted by the theoretical work. This work is valuable in two aspects. For one thing, it proves for the first time that ELF magnetic fields can induce the uptake of intracellular calcium levels in osteoblasts, which will contribute to the exploration of the molecular mechanisms of EMF effects on bone cells. For another, it provides an example for finding the quantitative map of EMF effects, which has a great reference value for clinical applications of EMF.更多还原