【作者】 刘颖刚；

【导师】 赵俊军；

【作者基本信息】 大连医科大学 ， 病理学与病理生理学， 2010， 硕士

【摘要】 目的：研究肿瘤抑制基因PTEN和凋亡抑制基因Survivin在膀胱尿路上皮癌中的蛋白表达情况,探讨二者与膀胱尿路上皮癌的分级、分期及预后的相关性和临床意义。方法：应用免疫组织化学SP染色方法检测60例膀胱尿路上皮癌及10例正常膀胱粘膜组织中PTEN蛋白和Survivin蛋白的表达。采用SPSS13.0软件包进行统计学分析。结果：(1)PTEN蛋白在10例正常膀胱组织中均有广泛黄染,均呈阳性表达,8例着色于细胞核,2例着色于细胞质。在60例膀胱尿路上皮癌组织中有21例阳性表达。PTEN蛋白在膀胱尿路上皮癌中的表达与在正常膀胱粘膜中的表达比较差异有明显统计学意义(P<0.01)。PTEN蛋白在膀胱尿路上皮癌的低级别与高级别、非浸润性与浸润性、初发与复发之间等各组之间表达差异有统计学意义(P<0.05)。在单发与多发、无淋巴结转移和有淋巴结转移之间表达差异无统计学意义(P>0.05)。PTEN蛋白阳性表达率与膀胱尿路上皮癌组织的分级、分期和肿瘤复发呈负相关显著性差异。(2)Survivin蛋白在10例正常膀胱组织中呈阴性表达。在60例膀胱尿路上皮癌组织中有42例Survivin蛋白着色于细胞核。Survivin蛋白在膀胱尿路上皮癌中的表达与在正常膀胱粘膜中的表达比较差异有明显统计学意义(P<0.01)。Survivin蛋白在膀胱尿路上皮癌组织的低级别与高级别、非浸润性与浸润性、单发与多发、初发与复发等各组之间表达差异均有统计学意义(P<0.05)。在无淋巴结转移和有淋巴结转移之间表达差异无统计学意义(P>0.05)。Survivin蛋白的阳性表达率与膀胱尿路上皮癌组织的分级、分期、肿瘤数量和肿瘤复发呈正相关显著性差异。(3)PTEN蛋白在膀胱尿路上皮癌的阴性表达与同时存在的Survivin蛋白阳性表达之间有明显统计学意义(P<0.01)。呈明显负相关显著性差异。结论：PTEN蛋白在膀胱尿路上皮癌和正常膀胱组织的阳性表达呈明显负相关,Survivin蛋白则呈明显正相关。PTEN和Survivin蛋白的表达与膀胱尿路上皮癌的生物学行为关系密切,可协同参与膀胱癌的生物学行为。PTEN蛋白的阳性表达与膀胱尿路上皮癌的恶性程度、分期及预后大致呈负相关,Survivin蛋白在膀胱尿路上皮癌中异常表达具有普遍性,其阳性表达与膀胱尿路上皮癌的恶性程度、分期及预后大致呈正相关。PTEN和Survivin蛋白的阳性表达在膀胱尿路上皮癌中呈明显负相关。联合检测PTEN和Survivin蛋白对诊断膀胱尿路上皮癌、判断其恶性程度及预后有一定参考价值。更多还原

【Abstract】 Objective:To investigate the protein expression and interrelation between the tumor suppress gene PTEN and apoptosis suppress gene Survivin in bladder urothelial carcinoma as well as to investigate the relationship and clinic significance of PTEN, Survivin in grade, stage and prognosis in bladder urothelial carcinoma.Methods:The expression of PTEN and Survivin protein were detected by S-P immunohistochemistry technique in 60 cases of bladder urothelial carcinoma and 10 cases of normal bladder mucosa. statistics analysis was progress with SPSS13.0 package.Results:(1) All 10 cases normal bladder tissues had positive express-ion of PTEN.8 cases colored caryon and 3 cases colored cytoplasm.21 positive expression in 60 cases bladder urothelial carcinoma. There was obviously statistically significantly differences of PTEN protein positive between bladder urothelial carcinoma and nomal bladder tissues (P<0.01). There was statistical significance in lower grade and higher grade, non-invasive and invasive, primary and recurrent cases(P<0.05). There was no statistical significance in single occurrence and multi-occurrence, non-lymphoid node metastasis and lymphoid node metastasis cases(P>0.05). The higher the grade, stage and recurrent of tumor was,the lower the expression of PTEN protein. (2) All 10 cases normal bladder tissues had not positive expression of Survivin protein.42 cases Survivin protein colored caryon in 60 cases bladder urothelial carcinoma. There was obviously statistically significantly differences of Survivin protein positive between bladder urothelial carcinoma and nomal bladder tissues(P<0.01).There was statistical significance in lower grade and higher grade, non-invasive and invasive, single occurrence and multi-occurrence, primary and recurrent cases (P<0.05). There was no statistical significance in non-lymphoid node metastasis and lymphoid node metastasis cases(P>0.05). The higher the grade,stage,quantity, and recurrent of tumor was,the higher the expression of Survivin. (3) There was obviously statistically significantly differences between PTEN protein negative expression and simultaneous Survivin protein positive expression in bladder urothelial carcinoma (P<0.01).Conclusion:The positive expression of the PTEN protein was a obviously negative correlation between in urothelial carcinoma of the bladder and in normal bladder tissues.The close relationship was found between the abnormal expression of PTEN, Survivin protein and the behavior in biology of the urothelial carcinoma of the bladder. PTEN and Survivin had the synergism.There was a negative correlation between the positive expression of the PTEN protein and malignancy degree, stage and prognosis of urothelial carcinoma of the bladder. The abnormal expression of Survivin is common in urothelial carcinoma of the bladder and there was a positive correlation between the positive expression of the Survivin protein and malignancy degree, stage and prognosis of urothelial carcinoma of the bladder.The expression of PTEN and Survivin protein presented significantly negative correlation.The coexpression of survivin and PTEN had invariably reference value in diagnosis, malignant degree and prognosis in urothelial carcinoma of the bladder.更多还原