【作者】 唐娜；

【导师】 郑建斌；

【作者基本信息】 西北大学 ， 分析化学， 2010， 硕士

【摘要】 过氧化氢(H2O2)电化学生物传感器因其简单,且对H2O2有高选择性和高灵敏性而得到广泛应用。本论文采用Au-Pt-PANI NPs、Fe3O4-PpPD NPs等纳米复合材料,构置了三种H202电化学生物传感器,研究了血红蛋白酶(HB)的电化学行为。该研究具有一定的科学意义和应用价值。全文共分三章,主要内容如下：1、综述了纳米材料和氧化还原蛋白质(酶)在H2O2电化学传感器中的应用情况,应用参考文献116篇。2、分别以CHIT-Au-Pt-PANI NPs、CHIT-Fe3O4-PpPD NPs作为固载膜,研究了血红蛋白酶(HB)在复合膜中的直接电化学行为,并建立了检测H2O2的分析方法。研究表明,CHIT-Au-Pt-PANI NPs、CHIT-Fe3O4-PpPD NPs复合膜能够提高氧化还原蛋白(酶)的直接电子传递速率,保持蛋白质(酶)的生物活性,并增强生物传感器的稳定性。这些研究工作对纳米复合材料在H2O2电化学生物传感器领域的构置和应用具有重要意义。3、利用辣根过氧化酶(HRP)催化H2O2的反应从而诱导苯胺以SWNTs为模板发生聚合反应,构置了一种基于酶催化体系新型的H2O2电化学生物传感器。研究结果表明,采用生物催化诱导体系所构置的检测H2O2的电化学生物传感器具有易操作、选择性好、灵敏度高等特点。该研究工作不仅扩展了生物催化在纳米材料合成领域的应用,而且为构置检测H202的电化学生物传感界面提供新方向。更多还原

【Abstract】 Hydrogen peroxide (H2O2) electrochemical biosensors have been widely used because of its simple fabrication, inherent high selectivity and sensitivity. In this thesis, we composed three kinds of hydrogen peroxide electrochemical biosensors using Au-Pt-PANI NPs or Fe3O4-PpPD NPs composite nanomaterials, which are used to investgate the electrochemistry behavior of Heme Oxygenase. The research has certain scientific significance and application value. The thesis consists of three chapters. The author’s main contributions are summarized as follows:1、Overview the application of nanomaterials and redox proteins (enzymes) in H2O2 electrochemical biosensors which are from 116 references.2. Study the direct eletrochemistry of hemoglobin (HB) by CHIT-Au-Pt-PANI NPs and CHIT-Fe3O4-PpPD composite nanomaterial films and structured two novel electrochemical biosensors for the determination of H2O2. The results indicated that CHIT-Au-Pt-PANI NPs and CHIT-Fe3O4-PpPD composite nanomaterial films with good biocompatibility can not only accelerate the direct electron transfer of redox proteins (enzymes) but also improve the stabilities of the biosensors. It can be conclude that the present studies extended the application of nanomaterials in novel electrochemical biosensors.3. Fabracation of a novel H2O2 biosensor based on enzymatically induced the deposition of electroactive polyaniline (PANI) at the horseradish peroxide (HRP)/aligned single-wall carbon nanotubes (SWCNTs) modified Au electrode, and investigated its electrochemical behaviors. The electrochemical impedance spectroscopy of the biosensor confirmed that the formation of PANI on SWCNTs through the HRP catalytic reaction. In the biosensing system, a highly simple, sensitive and selective biosensor for the detection of the H2O2 was achieved in connection with the biocatalytic deposition of PANI. The above studies may provide a general platform for the construction of bioelectronic devices and the design of novel electrochemical biosensors with other bionano-systems.更多还原