【作者】 陈琪峰；

【导师】 周永庆； 黄红光；

【作者基本信息】 浙江大学 ， 外科学， 2010， 硕士

【摘要】 目的研究胶质纤维酸性蛋白(Glial Fibrillary Acidic Protein, GFAP)、S100和Ki-67在人脑胶质瘤肿瘤细胞中的表达,探讨其表达程度同胶质瘤分级之间的关系及其临床意义。方法收集统计浙江大学医学院附属第一医院神经外科2009年3月至2010年3月住院并行手术治疗的32例胶质瘤患者肿瘤切除标本的病理报告及GFAP, S100及Ki-67的免疫组织化学检查结果。结果GFAP, S100和Ki-67三者在各级别的胶质瘤中均有表达。其中GFAP在各级胶质瘤细胞中的总阳性率高达96.9%；S100在高级别胶质瘤中的表达水平低于其在低级别胶质瘤中的表达水平(p<0.05)；而Ki-67在高级别胶质瘤中的表达水平高于其在低级别胶质瘤中的表达水平(p<0.05)。结论GFAP, S100和Ki-67的免疫组化阳性结果均可作为胶质瘤病理诊断的重要依据。其中S100和Ki-67二者的表达水平可用于辅助判断胶质瘤的等级。而Ki-67的表达水平能较准确的反应胶质瘤肿瘤细胞的增殖水平,对判断胶质瘤患者的预后具有重要的参考价值。更多还原

【Abstract】 ObjectiveTo examine the expression of GFAP, S100 and Ki-67 in human glioma, to discuss the value of GFAP, S100 and Ki-67 in histological grade and evaluate the clinical significance of them.MethodsHE and immunohistochemical staining technique was used to detect the expression of GFAP, S100 and Ki-67 in 32 patients, who were operated in the 1st Affiliated Hospital of Zhejiang University, College of Medicine, from March 2009 to March 2010. The results of HE and immunohistochemical staining are collected from the patients’ pathological reportResultsGFAP, S100 and Ki-67 express in all different grades of human glioma. The total positive rate of GFAP in all peitients is 96.9%. The expression level of S-100 was decreased with the increased grade of malignancy (p<0.05), while the expression level of Ki-67 was increased (p<0.05).ConclusionThe expression of GFAP, S100 and Ki-67 is very important to the pathological diagnosis of human glioma. The expression of S100 and Ki-67 are closely related with pathologic grade of glioma. And the Ki-67’s expression level correlates significantly with proliferating activity of glioma cell, which effects the peitients’prognosis.更多还原