【作者】 徐愔烨；

【导师】 郑伟；

【作者基本信息】 浙江大学 ， 临床医学， 2010， 硕士

【摘要】 目的：探讨HPV L1蛋白及IL-2在慢性宫颈炎、不同级别宫颈上皮内瘤变及宫颈鳞癌中的表达及其对宫颈病变预后的指导意义。方法：采用免疫组化S-P法检测22例慢性宫颈炎、24例宫颈上皮内瘤变Ⅰ级(CINⅠ)、28例宫颈上皮内瘤变Ⅱ级(CINⅡ)、37例宫颈上皮内瘤变Ⅲ级(CINⅢ)及13例宫颈鳞癌(SCC)组织中HPV L1蛋白及IL-2的表达。结果：随着宫颈上皮内瘤变级别的递增,HPV L1蛋白的表达逐渐降低,其在CINⅠ、CINⅡ及CINⅢ中的阳性表达差异有显著性意义(P<0.05)；IL-2在慢性宫颈炎组织及CINⅠ的表达明显高于CINⅡ、CINⅢ及SCC组织中的表达,差异有显著性意义(P<0.05)；在宫颈上皮内瘤变组织中,HPV L1与IL-2的表达呈正相关(P<0.05)；IL-2(-)／HPV L1(-)在CINⅠ、CINⅡ、CINⅢ及SCC中所占比例分别为4.17%、28.57%、59.46%及92.31%,其表达与宫颈病变严重程度呈正相关；而IL-2(+)／HPV L1(+)在CINⅠ、CINⅡ、CINⅢ及SC中所占比例分别为62.5%、25.00%、8.11%及0%,其表达与宫颈病变严重程度呈负相关。结论：HPV L1蛋白及IL-2的表达均随着宫颈病变严重程度的增加而减少,两者在宫颈疾病的发生发展中具有重要意义；L1蛋白和IL-2的检测对宫颈疾病的预后具有指导意义,IL-2(-)／HPV L1(-)者其病变进展的可能性较大,而IL-2(+)／HPV L1(+)者其病变则趋向于维持或消退。更多还原

【Abstract】 Objective:To elucidate the relation between HPV L1 capsid protein and IL-2 expressions in different lesions of cervix, and to explore their prognostic significance in cervical lesions.Methods:The expression of HPV L1 capsid protein and IL-2 were measured by S-P immunohistochemistry, respectively, in 22 cases of chronic cervicitis,24 cases of CINⅠ, 28 cases of CINⅡ,37 cases of CINⅢand 13 cases of cervical squamous cell carcinoma(SCC).Results:The expression of HPV L1 protein decreased as the cervix lesions progressed, and there was notably difference in CINⅠ, CINⅡand CINⅢ(p<0.05). The expression of IL-2 in chronic cervicitis and CINⅠwas remarkably higher than that in CINⅡ, CINⅢand SCCs (p<0.05). The expression of IL-2 was positively correlated with the expression of HPV L1 protein in tissues of CIN (p<0.05). The relation between HPV L1 protein and IL-2, IL-2(-)/L1(-)cases represented 4.17% of CINⅠ,28.57% of CINⅡ, 59.46% of CINⅢ, and 92.31% of SCCs, it was positively correlated with the severity of cervical lesions; whereas IL-2(+)/L1(+)cases were negatively correlated with the severity of cervical lesions, it represented 62.5% of CINⅠ,25.00% of CINⅡ,8.11% of CINⅢ, and 0% of SCCs.Conclusion:Expression of HPV L1 capsid protein and IL-2 decreased with lesion progression from chronic cervicitis to CIN and SCCs, they play an important role in the development of cervical intraepithelial dysplasias. The correlation between L1 and IL-2 expressions suggests that IL-2(-)/L1(-)cases have the potential for progression, whereas IL-2(+)/L1(+)cases may be nonprogressive lesions or potentially in remission.更多还原