【作者】 钱哨；

【导师】 杨兴升；

【作者基本信息】 山东大学 ， 妇产科学， 2009， 硕士

【摘要】 目的探讨17β-羟基类固醇脱氢酶-1(17β-HSD-1)基因多态性与子宫内膜腺癌发病风险的关系,以期为子宫内膜腺癌的病因学研究和高危人群的筛查积累资料。研究方法运用等位基因特异性PCR(ASA-PCR)技术分别针对不同的SNP设计6对引物。以病例-对照研究,从41例(实验组)子宫内膜腺癌患者手术切除的新鲜组织标本及同时选取27例(对照组)非子宫内膜腺癌患者门诊刮宫后且经病理证实为正常子宫内膜的新鲜组织标本中提取基因组DNA作为PCR模板,通过PCR扩增,琼脂糖凝胶电泳,检测17β-羟基类固醇脱氢酶-1基因1004位、1322位和1954位点多态性。采用SPSS13.0软件Chi-Square(χ~2)进行数据分析比较对照组和子宫内膜腺癌组之间的差异。结果子宫内膜腺癌组与对照组17β-HSD-1基因1004位、1322位和1954位SNP表达无显著差别,P值分别是0.944、0.974、0.981。结论子宫内膜腺癌组与对照组17β-HSD-1基因1004位、1322位和1954位SNP表达无显著差别,以上位点基因多态性可能与子宫内膜腺癌病因学无关。更多还原

【Abstract】 Objective To investigate the correlation between the17β-HSD-1 gene polymorphisms and risk for endometrial adenocarcinoma,in order to accumulate document for the etiology study and screening of endometrial adenocarcinoma.Methods Forty-one patients with endometrial adenocarcinoma were selected as study group and twenty-seven healthy women were selected as control group,the samples were collected and genome DNA were extracted as the template of PCR. The single nucleotide polymorphism of 17-beta-hydroxysteroid dehydrogenase-1 gene at sites 1004,1322 and 1954 were studied by allele-specific PCR(ASA-PCR) technique.SPSS13.0 software,Chi-Square(x~2)statistical were used to analysize the discrepancy between the group control and endometrial cancer.Result There was no significant discrepancy in SNP of 17β-HSD-1 gene at sites 1004,1322 and 1954 between endometrial adenocarcinoma and the control.Conclusion There is no significant discrepancy of SNP between at gene si-tes1004,1322 and 1954 of 17β-HSD-1 gene between group endometrial ad-enocarcinoma,and the control.It seems that there is no relationship betwe-en the polymorphism of 17β-HSD-1 gene with the etiopathogenisis of en-dometrial adenocarcinoma.Significance Study the relationship between the gene polymorphism of 17β -HSD with the etiopathogenisis of en-dometrial adenocarcinoma,conduce to further understand the molecule mechanism of tumour orignated,then offter new way for the endometrial adenocarcinoma’s prophylaxis,diagnosis and treatment.更多还原