【作者】 李玲；

【导师】 胡琼；

【作者基本信息】 遵义医学院 ， 妇产科学， 2009， 硕士

【摘要】 目的探讨遵义地区部分妊娠期肝内胆汁淤积症（intrahepatic cholestasis ofpregnancy,ICP）与人类白细胞抗原-DQA₁（human leucocyte antigen-DQA₁,HLA-DQA₁）等位基因多态性的关系,寻找遵义地区部分ICP患者的HLA-DQA₁易感基因或保护基因,为ICP的预防及治疗提供一定的依据。方法对研究对象抽取静脉血,提取DNA,采用聚合酶链反应—序列特异性引物（PCR-SSP）法研究遵义地区20个ICP孕妇和10个正常孕妇的HLA-DQA₁等位基因的多态性。结果所有标本共检出11种HLA-DQA₁基因表型,ICP患者HLA-DQA₁*0302基因频率高于正常孕妇（34.21%与15.79%）,但二者差异无显著性（P＞0.05）。结论HLA-DQA₁等位基因可能与遵义地区部分ICP患者的发病无明显关联性。更多还原

【Abstract】 Objective To investigate the relationship between human leucocyte antigen -DQA₁ Polymorphism distribution in intrahepatic cholestasis of pregnancy in zun yi district.Methods The HLA-DQA₁ typing were analysed in blood samples using polymerase chain reaction with sequence-specific primer（PCR-SSP）in twenty patients and ten healthy pregnant women.Results Eleven types of HLA-DQA₁ alleles were detected. Frequency of HLA-DQA₁ * 0302 in ICP is more than healthy pregancy（34.21%,15.79 %）,but the analysis showed no statistically significant difference between the two groups （P＞0.05）.Conclusion It is unlikely that an HLA-DQA₁ allele is primarily associated with susceptibility or resistance to ICP in Zunyi district.更多还原