体外动态学刺激对SD幼鼠前脂肪细胞活力、增殖、凋亡及TNF-a表达释放影响的研究

【作者】 谢西梅；

【导师】 陈波；

【作者基本信息】 贵阳中医学院 ， 针灸推拿学， 2009， 硕士

【摘要】 目的:探讨动态力学刺激对SD幼鼠前脂肪细胞的活力、增殖、凋亡及肿瘤坏死因子(TNF-α)表达释放影响的研究,为推拿按摩治疗青少年单纯性肥胖和肥胖并发症的现代医学细胞生物学机制提供理论与实验依据。方法:体外培养SD幼鼠前脂肪细胞,从细胞生物力学角度,模拟推拿治疗的按摩作用方式,对细胞实施动态力学刺激,观察细胞形态学变化,检测细胞活力、细胞增殖指数和细胞凋亡率的变化,并观测动态力学刺激对细胞TNF-α表达释放的影响。结果:(1)实验中所采用的体外动态力学刺激未对前脂肪细胞的细胞形态产生明显影响。(2)动态力学刺激抑制了前脂肪细胞的活力和增殖活性,而对前脂肪细胞的凋亡无显著性影响。(3)动态力学刺激抑制了前脂肪细胞TNF-α的释放表达。结论:推拿按摩治疗青少年单纯性肥胖和肥胖并发症的作用机制一方面可能通过抑制前脂肪细胞的活力和增殖,使前脂肪细胞向脂肪细胞转化率降低而达到;另一方面可能通过抑制前脂肪细胞TNF-α的释放表达,降低脂肪细胞源性细胞因子的病理作用而实现。更多还原

【Abstract】 Objective: From the mechanics perspective of cell biology, in vitro simulation of dynamic mechanical massage to stimulate the form of SD rats to explore the vitality of preadipocyte, proliferation, apoptosis and tumor necrosis factor (TNF-а) are released on the impact of the expression for Massage treatment of adolescents with simple obesity and obesity complications of modern medical cell biology mechanism based on theory and experiment.Methods: healthy SD rats in vitro culture of pre-obese fat cells, in different frequencies of vitro preadipocyte implementation of dynamic mechanical stimulation, observed changes in cell morphology; detect cell viability, cell proliferation index (PI) and apoptosis rates, and observation of dynamic mechanical stimulation on cell expression of TNF-аrelease.Results: (1) used in experiments, the effect on cell morphology did not preadipocyte in different frequencies of vitro dynamic mechanical stimulation. (2) In different frequencies of vitro dynamic mechanical stimulation, the preadipocyte viability and proliferation of preadipocyte apoptosis have no significant effections. (3) In different frequencies of vitro inhibition of the pre-fat cells release and the expression of TNF-аare expressed by the dynamic mechanical stimulation.Conclusions: Massage therapy adolescents with simple obesity and obesity complications mechanism on the one hand, probably by inhibiting preadipocyte viability and proliferation, so that preadipocyte to adipocyte conversion rate decreased to reach, on the other hand, probably through the dynamic mechanical stimulation, the preadipocyte release of TNF-аexpression are inhibited, reducing insulin resistance and implementation.更多还原