【作者】 朱利勇；

【导师】 钟狂飚；

【作者基本信息】 中南大学 ， 外科学， 2009， 硕士

【摘要】 目的:研究粘着斑蛋白(Vinculin,VCL)、雄激素受体(AndrogenReceptor,AR)、无机焦磷酸酶(Inorganic pyrophosphatase,PPA1)三因子在良性前列腺增生(Benign prostatic hyperplasia,BPH)、前列腺癌(Prostate Cancer,PCa)中的表达情况,明确并分析其与前列腺癌不同临床分期、病理分级、PSA水平之间相互关系,为前列腺癌的发生、进展提供理论基础。方法:采用免疫组织化学方法检测18例BPH组织,38例PCa组织中VCL、AR、PPA1的表达。分析VCL、AR、PPA1在BPH、PCa中表达差异;在PCa不同临床分期、病理分级、初次PSA检测水平上VCL、AR、PPA1表达情况;VCL、AR、PPA1三者之间相关性。结果:1)VCL、AR、PPA1在BPH组织中阳性表达率分别为28%、56%、22%,在PCa组织中分别为58%、53%、39%;VCL在PCa中表达较BPH组织增多(P＜0.05),AR、PPA1表达在BPH、PCa中无明显差异(P＞0.05)。2)VCL、AR在PCa组织中表达与肿瘤临床分期、病理分级密切相关(P＜0.05),与PSA值无明显关系(P＞0.05);PPA1在PCa组织中表达与肿瘤临床分期、病理分级、PSA均无明显相关性(P＞0.05)。3)VCL、AR两者在PCa组织中表达存在正相关(r=0.489,P=0.002＜0.05);PPA1在PCa中表达与VCL、AR无相关性存在(P＞0.05)。结论:1) VCL在BPH、PCa表达具有差异性,可能作为前列腺良、恶性鉴别诊断的一个潜在指标;2) AR、VCL随PCa进展表达逐步下降,两者存在正相关,两者结合可能成为前列腺癌进展的诊断、预后评价指标;3) PPA1非评价PCa进展有效指标。更多还原

【Abstract】 Objective:To study the expression of three factors,Vinculin(VCL), androgen Receptor(AR),and inorganic pyrophosphatase(PPA1) in benign prostatic hyperplasia(BPH),and prostate cancer(PCa);To clear and analyze the mutual relationship between the three factors and prostate cancer’s different clinical stages,histological grade,and PSA level;To provide a theory for the occurrence and the development of PCa.Methods:To detected the expression of VCL,AR,and PPA1 in 18 cases of BPH tissue and 38 cases of PCa tissue by immunohistochemistry; To analysis the differences of VCL,AR,PPA1 in the expression of BPH and PCa;To detected the expression of VCL,AR,and PPA1 in prostate cancer’s different clinical stages,histological grade,and PSA level;To analysis the correlation of VCL,AR,and PPA1.Results:1) The positive expression rate of VCL,AR,PPA1 were 28%,56%,22%in BPH tissues,58%,53%,39%in PCa tissues.The positive rate of VCL in PCa was more than in BPH tissues(P＜0.05),the positive rate of AR and PPA1 were no significant difference in BPH and PCa(P＞0.05).2) There was a close relationship between the expression of VCL, AR and tumor clinical stage,pathological grade in PCa tissue(P＜0.05), but without significant relationship in the value of PSA(P＞0.05).The expression of PPA1 was not significant correlation with the tumor clinical stage,pathological grade and the value of PSA(P＞0.05).3) There was a positive correlation between the expression of VCL and the expression of AR in PCa tissue(r= 0.489,P= 0.002＜0.05).There was no correlation in the expression of PPA1,VCL,and AR in PCa tissue (P＞0.05).Conclusions:1) The differences between the expressions of VCL in BPH and PCa predicted VCL could be used as an indicator for the differential diagnosis of benign and malignant prostate disease;2) The expression of AR and VCL reduced gradually with the progression of PCa and there was a positive correlation between them. The combination of the two indicators could be an indicator of diagnosis and prognosis to evaluate the development of PCa;3) PPA1 wasn’t an effective indicator to evaluate the progression of PCa.更多还原