【作者】 张阳；

【导师】 张伟；

【作者基本信息】 山东中医药大学 ， 中医内科学， 2009， 硕士

【摘要】 目的:通过实验室模拟相应环境,对健康大鼠进行一定的刺激,以及对相关指标检测的评价,对中医经典理论“形寒饮冷则伤肺”的实质进行更深层次地探讨。方法:选取健康Wistar雄性大鼠56只,随机分为以下4组:空白对照组、形寒组、饮冷组、形寒饮冷组,每组14只,将各组大鼠置于相应条件的调温箱或者给予相应的刺激,期间观察大鼠的一般情况变化及皮肤、毛发、饮食、便质、行为等变化,7日后采集标本,观测其肺组织病理改变,放免法检测血清CRP、肺组织匀浆IL-8的含量变化,酶免法检测β防御素在肺组织中的表达,原位杂交法检测TGFβ1-mRNA在肺组织及支气管上皮中的表达。应用SPSS13.0统计软件对实验数据进行统计学处理,并分析结果。结果:形寒组、饮冷组、形寒饮冷组大鼠肺组织形态发生了明显改变,大鼠血清CRP升高,大鼠肺组织匀浆中IL-8含量升高、β防御素含量降低,以及TGFβ1-mRNA表达升高,其中形寒饮冷组最为显著,形寒组次之,饮冷组再次之。结论:从多层次多角度进一步证实了“形寒”、“饮冷”与肺脏损伤之间存在的密切相关性,实验证明单纯“饮冷”亦可造成肺脏损伤,从一定程度上揭示了“形寒饮冷则伤肺”理论的内涵与实质,为拓展该理论的现代研究与应用范围提供了一定的思路、方法与依据。更多还原

【Abstract】 AIM: To deep discuss Chinese Medicine classical theory“eating cold food in cold temperature will hurt the lung”through simulated atmosphere in the lab, and appraise concerned guidelines’inspection.Method: Choose 56 healthy Wistar male rats, and divide them into 4 groups randomly, they are: control group, cold-temperature group, cold food-eating group, eating cold food in cold temperature group; 14 ones for each group. Put them into different temperature-controlled boxes or give them different stimulation. During this period, observe their changes, such as their skin, fur, food-eating, stool quality and behaviors. After 7 days, collect samples, observe their pathological changes in pulmonary tissue; exam serum CRP by radioimmunoassay method, the changes of pulmonary tissue homogenate IL-8 contents; examβalexin’s expression in pulmonary tissue through enzyme immunoassay; exam TGFβ1-mRNA’s expression in pulmonary tissue and bronchus through Situ Hybridization Method. Making statistical treating to the experimental data by applying SPSS13.0 statistics software, and analyze the results.Result: The rats in cold-temperature group, cold food-eating group and eating cold food in cold temperature group, whose pulmonary tissues have obvious changes; i.e. serum CRP rose, IL-8 contents in rats’pulmonary tissue homogenate increased,βalexin contents decreased, and the expression of TGFβ1-mRNA increased; eating cold food in cold temperature group has most obvious symptom; cold-temperature group has less obvious symptom; the lest obvious symptom for cold food-eating group.Conclusion: It’s proved further that“cold-temperature”and“cold food-eating”have close pertinence with lung hurt from multi-layer and multi-angle observation. The experiment also proved that single“cold food-eating”also can case lung hurt; it also reveals the connotation and essence of“eating cold food in cold temperature will hurt the lung”, so as to supply some ways or gist for developing the research and applied scope for this theory.更多还原