【作者】 管雪梅；

【导师】 周凤琴；

【作者基本信息】 山东中医药大学 ， 中药学， 2009， 硕士

【摘要】 目的:研究忍冬叶的生药学特征和内在质量,为忍冬叶药材标准的制定提供实验数据和科学依据。方法:采用生药学的方法,对忍冬叶原植物进行了形态描述,对忍冬叶药材的外观性状和显微特征进行了观察研究。参考《中国药典》关于中药材质量标准的有关规定,对忍冬叶药材的水分、总灰分和酸不溶性灰分进行了检查;根据忍冬叶已知化学成分的性质,测定了水溶性和醇溶性浸出物的含量;采用高效液相色谱法测定了绿原酸的含量;采用傅立叶变换红外光谱法制定了忍冬叶红外指纹图谱;采用高效液相色谱法,制定了忍冬叶的指纹图谱,并同时测定了木犀草苷的含量。结果:提出了忍冬原植物形态和忍冬叶性状鉴别特征,制定了忍冬叶优质药材的外观标准;研究出忍冬叶的组织结构以及粉末鉴别特征。初步确定了忍冬叶药材中水分含量不得过12.0%,总灰分不得过8.0%,酸不溶性灰分不得过0.80%的检查标准。初步制定了水溶性浸出物不得少于27.0%,醇溶性浸出物含量不得少于24.0%,绿原酸含量不得少于0.80%,木犀草苷含量不得少于0.12%的内在质量标准。初步确定了忍冬叶红外指纹图谱和高效液相指纹图谱。结论:为忍冬叶药材的准确鉴定和质量标准的制定提供了科学依据和资料,也为忍冬叶的开发应用研究奠定了基础。更多还原

【Abstract】 Objective: Study the pharmacognostical characteristics and inherent quality of medicinal materials of the leaves of Lonicera japonica, provide experimental data and scientific evidence for establishing the quality standards.Methods: First using the Pharmacognosy methods, the external appearance of original plant, appearance of characters and microscopic characteristics of medicinal materials of the leaves of Lonicera japonica were investigated. Then, according to the Pharmacopoeia of the Peple’s Republic of China (2005 Edition) the water, ash and extract contents of the medicinal materials of the leaves of Lonicera japonica were measured, and the contents of Chlorogenic acid and luteolin glycosides were determined with the HPLC. Last,the FTIR and HPLC were adopt to establish the fingerprint of the leaves of Lonicera japonica.Results: The external appearance of original plant and the appearance of characters were submitted, and high quality standard of characteristics of leaves of Lonicera japonica were established. The organizational structure and powder identification characteristics of leaves of Lonicera japonica were also assayed, The quality standard of leaves of Lonicera japonica requires as the following: water content should be not more than 12.0%, total ash not more than 8.0%, acid-insoluble ash not more than 0.80%, water-soluble extract not less than 27.0%,alcohol extract not less than 24.0%, chlorogenic acid not less than 0.80%, luteolin glycosides not less than 0.12%.The FTIR and HPLC fingerprinting were initially set up.Conclusions: The research results provided theoretical evidence and data for accurately identifying and establishing the quality standard of the leaves of Lonicera japonica, and for utilizing the leaves of Lonicera japonica.更多还原