【作者】 华梅；

【导师】 林思祖；

【作者基本信息】 福建农林大学 ， 园林植物与观赏园艺， 2009， 硕士

【摘要】 多花相思(Acacia floribunda (Vent.) Willd.)自然分布于澳大利亚昆士兰、新南威士和维多利亚等州,适应性较强,有较高的观赏价值。本论文以自然状态下多花相思的种子为基本材料,从无菌体系的建立、继代培养、生根诱导、炼苗移栽等各个环节展开多花相思无菌体系的建立及快繁技术的研究;同时以多花相思种子苗下胚轴以上部分为材料,进行愈伤组织的诱导,并从愈伤组织分化出苗。主要研究结果如下:1.多花相思无菌体系的建立。采用1.84g/mL浓硫酸处理种子10min效果最好,发芽率可达90%。然后用75%酒精浸泡10s,0.1%升汞浸泡15min,无菌水冲洗7遍,可以使污染率控制在3%,最后接种到MS培养基中,生长状况良好。2.采用正交试验设计,考虑不同植物生长调节剂对多花相思小苗增殖的影响,筛选出最佳一代培养基为MS+KT0.4mg/L+IBA0.5mg/L +BA0.5mg/L +蔗糖30g/L,30d后增殖数可以达到2.914;最佳二次继代培养基为MS培养基+KT0.3mg/L+BA1.0mg/L+IBA1.5mg/L +AC1.2g/L +蔗糖30g/L;30d后增殖数可以达到4.179;最佳三次及多次继代培养基为MS+BA0.2mg/L+IBA0.3mg/L +蔗糖20g/L。30d后增殖数可以达到3.309。3.多花相思小苗生根诱导。采用正交试验设计,考虑不同种类和浓度生长素对多花相思小苗生根的影响,筛选出最佳生根培养基为MS+IBA0.3mg/L +IAA0.1mg/L +蔗糖30g/L。4.多花相思组培苗的炼苗和移栽。实验结果表明:黄心土:泥炭土=2:1的效果最好,成活率达到85.5%。5.多花相思愈伤组织体系的建立。以多花相思种子苗下胚轴以上部分为愈伤组织诱导材料,筛选出愈伤组织的最佳诱导培养基为MS+ BA1.0mg/L+KT0.6mg/L +IBA0.6mg/L+蔗糖30g/L。愈伤组织的最佳继代培养基为MS+ BA1.0mg/L+KT1.0mg/L +IBA0.6mg/L+蔗糖30g/L。当光照强度为1200Lx时,愈伤组织长势最好。6 .多花相思愈伤组织培养再生植株。以多次继代的愈伤组织为基本材料,在MS+ TDZ0.01mg/L+NAA0.2mg/L +蔗糖30g/L培养基中,可使愈伤组织分化率达到89%,将分化愈伤组织转接在MS+ TDZ0.01mg/L+NAA0.2mg/L +蔗糖30g/L培养基上,再生率为98%。在1/2MS +IBA0.5mg/L+NAA0.1mg/L+AC1.2g/L+20g/L蔗糖培养基上,30天后,小苗增殖数为1.41。更多还原

【Abstract】 Acacia floribunda (Vent.) Willd. grows in Queensland Australia, New South , WalesVictoria ect. It has good adaptation, high appreciation value. This experiment chose Acacia floribunda (Vent.) Willd.’s seeds which grew in natural state as basic material, researching about every steps of seeds axenic bourgeon, proliferation, root induction, transplantion etc. At the same time, The parts which above the seed’s hypocotyl were used as the initial material for the inducement of calli, calli systems were established, and polarized seedling from calli. The main results were described as follows:1. The establishment of Acacia floribunda (Vent.) Willd . axenic system.Seed capsule was rigid and compact, deep in oil of vitriol for 10 minutes, reached 90% burgeon rate. The result showed that the method which 75% alcohol disinfected 10 seconds first, then deeped in 0.1%HgCl2 for 15 minutes, and axenic water washed 7 times last was best. It can control the pollution rate below 3%. Next, the seeds were inocu ated to the medium of MS , kept vigorous growth of seeds.2. The proliferation of Acacia floribunda (Vent.) Willd. axenic seedlings. Considered the influence of different plant growth plant growth regulators on the proliferation, which orthogonal design, screened out the medium was MS supplemented with KT0.4mg/L, IBA0.5mg/L, BA0.5mg/L, sugar 30g/L, and the proliferation rate could reach 2.914 by 30d later. In the second repetitious cultivation, the medium was MS supplemented KT0.3mg/L,BA1.0mg/L ,IBA1.0mg/L, AC1.2g/L ,sugar 30g/L and the proliferation rate could reach 4.179 by 30d later. In the third and more times proliferation, the medium was MS+BA0.2mg/L +IBA0.3mg/L +sugar20mg/L and the proliferation rate could reach3.309 by 30d later.3. Rootage of Acacia floribunda (Vent.) Willd. Thinked about the influence of different kind and concontraction plant growth regulators on the axenic seedlings root rate, screened the medium was MS supplemented with IBA0.3mg/L, IAA0.1mg/L and sugar 30g/L.4. The transplantion of Acacia floribunda (Vent.) Willd. Analysis the results of the survival rate of the different Transplanted stroma of Acacia. f loribunda (Vent.) Willd., it showed that ocher:turf=2:1was best and the survival rate can reach 85.5%.5. The establishment of Acacia floribunda (Vent.) Willd. Calli system. Chose the parts which above seed’s hypocotyl as basic material for the inducement of calli, under the light, consider the influence by different kind and concentration of plant growth regulators and accretion, the medium was MS supplemented with BA1.0mg/L, KT 0.6mg/L, IBA0.6mg/L and sugar 30g/L,and the proliferation medium was MS supplemented with BA1.0mg/L,KT1.0mg/L, IBA0.6mg/L and sugar 30g/L .When the light was 1200lx, the calli grew well.6. The generation of calli. Under the light of 1200lx, considering the influence of plant growthplant growth regulators and accretion. MS medium supplemented with TDZ0.01mg/L,NAA0.2mg/L, and sugar 30g/L, the calli provenance could reach 89%.MS supplemented with TDZ 0.01 mg/L, NAA0.2mg/L, and sugar 30g/L. the regeneration rate of calli can reach 98%. MS supplemented withTDZ0.01mg/L,NAA0.2mg/L, and sugar 30g/L. the regeneration rate of calli can reach 98%. 1/2MS supplemented with IBA 0.5 mg/ L ,NAA0.1mg/L, AC1.2g/L and sugar 20g/L. Recorded 30d later, the proliferation rate could reach 1.41 .更多还原