【作者】 杨洋；

【导师】 刘大程；

【作者基本信息】 内蒙古农业大学 ， 预防兽医学， 2009， 硕士

【摘要】 本试验研究延胡索酸二钠与瘤胃素组合在高精料日粮条件下对慢性瘤胃酸中毒状态下瘤胃发酵功能和微生物数量的影响。采用体外批次培养研究在酸中毒条件下延胡索酸二钠(A)和瘤胃素(B)添加剂组合对瘤胃微生物体外发酵的影响,预设9种组合(详见表),瘤胃液由日粮比5:5的泌乳期奶山羊提供,批次底物精粗比为8:2,检测瘤胃发酵指标pH值、产气量、NH3-N浓度。通过体外法综合四项指标比较得出最适宜的莫能菌素和延胡索酸二钠组合剂量为第4组(34mg+10g)。选取6只处于泌乳期并安装永久性瘤胃瘘管的奶山羊作为试验动物,随机分为试验组和对照组。两组动物均采用逐渐提高日粮精料的方式诱导发生SARA。试验动物处于SARA后,试验组添加组合添加剂,对照组不添加。测定瘤胃发酵指标并通过16S rRNA探针杂交定量分析法结合传统厌氧培养法分析瘤胃内细菌的变化情况。结果表明,组合添加剂能显著提高瘤胃VFA浓度(P<0.05),并能稳定高精料条件下瘤胃pH值,使乳酸杆菌和牛链球菌数量减少(P<0.05),反刍兽新月单胞菌、埃氏巨型球菌和产琥珀酸丝状杆菌的数量增加(P<0.05)。结果提示,延胡索酸二钠和瘤胃素的联合使用可以改善SARA状态下的瘤胃发酵功能,并使乳酸产生菌减少而乳酸利用菌增加。更多还原

【Abstract】 The aim of this work was to evaluate the effect of ruminal fermentation and populations of total bacteria when the goat fed with disodium fumarate and monensin on the condition of subacute rumen acidosis(SARA). The ruminal bacteria fermented in vitro adding disodium fumarate(A) and monensin(B). There were 9 types of combination (chart 1-2), rumen fluid was gathered from lactating dairy goat fed with dietary ration 5:5,the ratio of concentrate to roughage in batch was 8:2,pH, gas production, NH3-N concentration and VFA were tested during ruminal fermentation. Though analysis of MFAEI, the best dose of combination of monensin and disodium fumarate was group 4.(Monensin 34mg + Disodium fumarate).The effect adding both disodium fumarate and monensin into diets on ruminal fermentation and bacteria amounts in dairy goats suffered from subacute rumen acidosis(SARA) were studied. Six lactating goats were induced in SARA by feeding concentrate daily in gradually increasing feeding regime, then the animals were randomly divided into two groups, the animals in test group were fed the combined additives, the animals in control group were not . The The ruminal fermentation parameters and the populations of total bacteria and lactate-metabolizing bacteria in SARA period were determined by the traditional roll-tube technique and 16SrRNA oligonecleotoide probes hybridization.The pH value and TVFA concentrations increased significantly(P<0.01), the populations of Lactobacillus and S.bovis decreased significantly (P<0.05), the quantities of S.ruminantium,M.elsdeni and B.succinogenes increased(P<0.05).The ruminal fermentation function could be improved by feeding the combined additives under SARA condition.The numbers of lactic acid - utilization increased and The populations of lactic acid- production decreased.更多还原