【作者】 孟祥伟；

【导师】 芒来；

【作者基本信息】 内蒙古农业大学 ， 动物遗传育种与繁殖， 2009， 硕士

【摘要】 胰岛素样生长因子Ⅰ(Insulin-like growth factor-Ⅰ)是近年来在家畜遗传育种中被广泛关注的候选基因之一。它与家畜的生长发育有着密切的关系。但国内外关于该基因是否影响中国蒙古马生长发育的研究开展却较少。本课题通过PCR-SSCP技术、PCR-RFLP技术对四个类型的地方品种中国蒙古马及引入品种纯血马、培育品种三河马共277个样品的胰岛素样生长因子Ⅰ基因的3′,5′端两个侧翼区及第三、四两个外显子进行了多态性进行了检测和序列比较分析,得出以下结论:(1)通过PCR-SSCP检测,在马的IGF-I第3、第4外显子区域,未检测到多态位点。但在IGF-Ⅰ基因3′侧翼区检测到多态位点,检测到AA、AB、BB三种基因型。并且该三种基因型和马的体尺指标具有相关性。(2)通过PCR-RFLP分析,采用3种限制性内切酶BsmaⅠ、DraⅠ、BamhⅠ进行酶切,在IGF-Ⅰ基因5′侧翼区并没有检测到多态位点。可能原因是所选择的酶恰好没有检测到,另外IGF-Ⅰ基因5′侧翼区序列相当保守。因此,此位点是否调控马的生长发育还需要进一步深入的研究。(3)不同类群马IGF-Ⅰ基因3′侧翼区位点的遗传信息参数分析结果表明:纯血马多态信息含量最高为0.589,乌审马最低为0.432,此位点遗传信息参数可能为马的分子遗传育种提供理论基础。更多还原

【Abstract】 Recently, Insulin-like growth factor-I is one of the most famous gene in heredity breeding area, which has nearly relationship with domestic animal growth. However, just a few researches that the gene how to modulate the development of Chinese Mongolia horse has been done. In this work, we used the methods of PCR-SSCP and PCR-RFLP, aligned the 3’and 5’ end flanking and 3rd and 4th exon sequence of IGF-I from 277 house samples(including 4 kinds of Chinese Mongolia house, TB house and breeded SH horse)and analyzing the gene polymorphisms, the results can be listed as follows :1) According to the PCR-SSCP result, the sequence of 3rd and 4th exon is more conserved than polymorphic. Analysis genotype and gene frequency of the IGF-I 3’end flanking, we found AA, AB and BB genotypes. These genotypes is concerned with the index of body size.2)During the PCR-RFLP analysis, we used BsmaI, DraI and BamHI restriction enzymes to examine the 5’end flanking polymorphisms and no difference was found. The reason may be that the restriction enzymes was not suitable. Therefore, whether the IGF-I 5’end flanking regulate house growth or not, we are still not sure.3) The analysis result of the polymorphism information content (PIC) of IGF-I 3’end flanking indicated that: SH horse has highest (0.589), WS horse has lowest polymorphism information conten(t0.432). The genetic information of this site could be a basic theory for horse molecular genetic breeding..更多还原