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羧甲基壳聚糖促牙周组织再生的毒理学研究
A Study of the Safety of N,O-Carboxymethty Chitosan Using in Periodontal Regeneration
【作者】 王训霞；
【导师】 张广耘；
【作者基本信息】 青岛大学 ， 牙周病学， 2009， 硕士
【摘要】 目的:研究羧甲基壳聚糖应用于牙周组织的安全性。方法:将32只昆明小鼠随机分为4组(每组8只),A为空白对照组,B为腹腔注射环磷酰胺阳性对照组,C、D为羧甲基壳聚糖梯度实验组,在手术骨缺损区置入梯度剂量的羧甲基壳聚糖。术后4周处死动物,应用彗星实验、嗜多染红细胞微核实验计算彗星尾数和微核率并进行统计学分析;收集牙周组织标本进行组织学观察。结果:A组、C组、D组间红细胞微核率及彗星尾数无显著差异(P＞0.05),且C组与D组间亦无统计学差异(P＞0.05),而B组的红细胞微核率及彗星尾数远高于其余3组(P＜0.01)。术后4周C、D组骨缺损区可见少量新骨形成,A组未见新骨形成。结论:羧甲基壳聚糖在该毒理学实验中未显示毒性结果,提示牙周组织局部应用羧甲基壳聚糖安全可靠;并具有促进牙周骨组织再生的作用。
【Abstract】 Objective:To study the safety of N,0-Carboxymethy chitosan using in periodontal regeneration.Method:32 mices were randomly divided into four groups with 8 rats in each group.Group A is negative group,B is positive group,C and D are different dose groups.N,0-Carboxymethy was implanted in mice’s periodontal tissue of group C and D.Observed it’s chronic and genetic toxicity by micronucleus,comet assay,tissue sections after 4 weeks.Results:The number of micronucleus rate and comet tail in group B are much higher than A,C,D(p＜0.01);obvious difference wasn’t exited between negative and test group(p＞0.05),moreover,low group C and D also exited little difference(p＞0.05).Conclusion:N,0-Carboxymethy chitosan doesn’t exit toxicity in appling to periodontal tis