【作者】 阿依古丽·哈里米西；

【导师】 单利；

【作者基本信息】 新疆医科大学 ， 肿瘤学， 2009， 硕士

【摘要】 目的:探讨溶骨性骨代谢生化指标血Ⅰ型胶原交联氨基末端肽(NTx)的定量测定对肺癌骨转移治疗的意义。本文用人肺小细胞肺癌细胞株H446种植于免疫缺陷小鼠制成小细胞肺癌骨转移裸鼠动物模型,通过观察NTx在氯膦酸二钠对小细胞肺癌骨转移荷瘤鼠的实验疗效中的变化,进一步探讨NTx定量测定预测氯膦酸二钠治疗小细胞肺癌骨转移的疗效的意义。方法:(1)将18只BALB/c-nu/nu雄性裸鼠随机分成正常组、实验组(予灌胃给药氯膦酸二钠)和对照组(予灌胃给药0.9%氯化钠注射液)。(2)将人肺小细胞肺癌细胞株H446种植于治疗组和对照组裸鼠右后肢胫骨,制成小细胞肺癌骨转移裸鼠动物模型。(3)细胞接种2周后利用放射学检查手段初步判定骨转移动物模型建立,始服药6周,第一次服药前及处死前半小时留血样各1次,每1周测体重,观察存活情况。(4)给药6周后处死裸鼠,所有裸鼠行放射学检查并取各裸鼠的右后肢行病理分析;用ELISA方法测定血NTx水平。结果:放射学及病理检查显示裸鼠接种H446细胞后引起明显的肿瘤性骨损伤;经氯膦酸二钠治疗后,实验组与对照组相比肿瘤进程有差异。实验组、对照组裸鼠血清NTx高于正常组裸鼠血清NTx ( P < 0. 01),实验组裸鼠经氯膦酸二钠血治疗前后血清NTx水平差别有统计学意义( P < 0. 01)。结论:实验证实定量测定NTx水平变化可用于预测氯膦酸二钠治疗小细胞肺癌骨转移的疗效。血清NTx对小细胞肺癌骨转移的诊断有重要参考意义,可协助及时诊断恶性肿瘤骨转移。且氯膦酸二钠可明显减轻小细胞肺癌骨转移程度。更多还原

【Abstract】 Objective: To establish a skeletal metastasis model of lung caner, and evaluate the prognosis effects of serum cross-linked N-telopeptide of typeⅠcollagen(NTx) in disodium clodronate against skeletal metastasis model of lung cancer.Methods:A skeletal metastasis model of lung caner was established by injection of small cell lung carcinoma line(SCLC) H446 cells into the right leg of nude mice, and disodium clodronate was given to the animals after radiologically small but de-fined osteolytic metastasis were observed. Both X-ray and histological assay were applied to diagnose establishment of bone metastasis. The levels of serum NTx were measured using ELISA. Results: Both X-ray detection and histological studies showed markedly injury after injection cells into the right leg of nude mice.The level of serum NTx in the nude mice with bone metastasis was significantly higher than in the nude mice without bone metastasis ( P < 0. 01), and it was also significantly different in the nude mice with disodium clodronate.Conclusion: The measurement of serum NTx is significant utility as biochemical markers of bone metastasis with malignant carcinoma and can be used to achieve a timely diagnosis for bone metastasis of the malignant tumor.And disodium clodronate may be a choice for the treatment of metastasis cancer in bone.更多还原