【作者】 黄萍；

【导师】 姚传义；

【作者基本信息】 厦门大学 ， 生物化工， 2008， 硕士

【摘要】 分子印迹聚合物是一种具有特异性分子识别能力的新型功能材料,被广泛应用于色谱分离、固相萃取、生物模拟传感器、膜分离等众多领域。以4-氨基安替比林为模板分子,十二醇/甲苯为溶剂,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,采用沉淀聚合法合成了一系列分子印迹聚合物,通过在水相中的结合实验研究了溶剂的种类和用量、聚合反应液中各组分的浓度对分子印迹聚合物识别能力的影响。在溶剂用量15mL,甲苯体积含量10%,模板分子浓度0.02mol/L,功能单体浓度0.1mol/L,交联剂浓度0.4mol/L,引发剂浓度0.002mol/L的条件下,制备出的分子印迹聚合物微球特异性识别能力最佳,对4-氨基安替比林及其结构类似物安替比林的分离因子为3.72。Scatchard分析表明,4-氨基安替比林与印迹聚合物之间存在2类不同的结合位点:高亲和力结合位点的平衡离解常数K_d=3.89μg/mL,最大表观结合常数Q_max=49.99μg/mg;低亲和力结合位点的离解常数K_d=84.03μg/mL,最大表观结合常数Q_max=11.69μg/mg。以壳聚糖为手性添加剂,利用毛细管电泳对色氨酸对映体进行拆分,考察了缓冲液的盐浓度、壳聚糖质量浓度、Cu²⁺浓度和缓冲液的pH值对分离过程的影响。实验结果表明,在盐浓度为5mmol/L,壳聚糖质量浓度为0.02%,Cu²⁺浓度为1.5mmol/L,缓冲液的pH为3.5的条件下,色氨酸对映体分离效果良好,分离因子为1.87,分离度为1.02,分离重复性良好。更多还原

【Abstract】 Molecular imprinting is a versatile and facile method for preparing synthetic polymers with predetermined molecular recognition properties.It can be employed in a variety of applications,such as chromatography separations,solid extractions, sensors,membrane separation and so on.In the first part of this study,molecular imprinting polymer microspheres with 4-aminoantipyrine（4-AP） as template were prepared by precipitation polymerization. The mixtures of dodecanol and toluene,methacrylic acid,ethylene glycol dimethacrylate were used as solvent,functional monomer,cross linker,respectively. The ability of the polymers to rebind 4-AP specifically in aqueous solutions was assessed.A best adsorption behavior and molecular selectivity occurred when 15mL solvent with 10%toluene was used and the concentrations of template,functional monomer,cross linker as well as initiator were 0.02mol/L,0.1mol/L,0.4mol/L, 0.002mol/L,respectively.The separation factor was up to 3.72 when antipyrine was used as the competing molecule.Scatchard analysis showed that the MIPs recognized 4-AP with two classes of binding sites.The dissociation constants and the maximum apparent binding capacities were K_d=3.89μg/mL,Q_max=49.99μg/mg for high affinity binding site and K_d=84.03μg/mL,Q_max=11.69μg/mg for low affinity binding site.In the second part of this study,chitosan,a cationic natural polymer,was employed as a chiral additive selector for enantioseparation of DL-tryptophan in capillary electrophoresis.The effects of several parameters on the ability of separation were studied.A best separation of tryptophan enantiomers was achieved by using 5mmol/L NaAc,0.02%（w/v） chitosan and 1.5mmol/L Cu²⁺ while the pH of the buffer was 3.5.The separation factor was 1.87 and the resolution was 1.02.The performance reproducibility was good.更多还原