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川芎嗪对哮喘大鼠GATA-3及Th2型细胞因子表达的影响
Effect of Ligustrazine on the Expression of GATA-3 and Th2 Cellular Factor in the Rat Asthma Model
【作者】 冯罡；
【导师】 陶晓南；
【作者基本信息】 华中科技大学 ， 内科学， 2007， 硕士
【摘要】 【目的】研究腹腔注射川芎嗪对大鼠哮喘模型的防治作用及机制。【方法】按常用方法制作大鼠哮喘模型并分为哮喘模型组(A组),川芎嗪治疗组(L组),地塞米松治疗对照组(D组)和正常对照组(N组)4组,每组8只。用酶联免疫吸附实验(ELISA)检测支气管肺泡灌洗液(BALF)中白介素-4(IL-4)和白介素-5(IL-5)的浓度。用HE染色的方法观察气道炎症变化。用免疫组化法研究肺组织GATA-3的表达。【结果】(1)L组BALF中IL-4和IL-5明显低于A组的IL-4和IL-5,差异有显著性(P<0.01),与D组无显著差异(P>0.05)。(2)L组肺组织GATA-3阳性细胞的光密度值(OD值)明显低于A组OD值,差异有显著性(P<0.01),与D组无显著差异(P>0.05)。【结论】腹腔注射川芎嗪可以抑制哮喘大鼠IL-4和IL-5的合成,其机制可能与其降低GATA-3在肺组织的表达,从而继发抑制Th2型免疫反应有关。
【Abstract】 【Objective】To investigate the prevention and treatment effect of intraperritoneal ligustrazine on bronchial asthma in the SD rat asthma model and the relevant mechanism.【Methods】The SD rat asthma model was made with classic method. All rats were divided into four groups, including asthma group (Group A, n=8), ligustrazine group (Group L, n=8), dexamethasone group (Group D, n=8), normal control group (Group N, n=8). The level of IL-4 and IL-5 in BALF were measured by ELISA. The airway inflammatory was observed with HE staining method. The expression of GATA-3 in pulmonary tissue was detected by means of immunohistochemicalstaining.【Results】(1) The level of IL-4 and IL-5 in BALF in Group L were much lower than those in Group A (P<0.01), there was no statistical significant difference between Group L and Group D (P>0.05). (2) The optical density of GATA-3 positive cells in pulmonary tissue in Group L was much lower than that in Group A ( P<0.01), there was no statistical significant difference between Group L and Group D(P>0.05)。【Conclusion】ligustrazine could lower the levels of IL-4 and IL-5 in the rat asthma model. The mechanism may be related to the action of ligustrazine to down-regulate GATA-3 expression in pulmonary tissue, resulting to suppression of Th2 reaction.