三七二醇预处理诱导脑缺血耐受及对GFAP和IL-1 β mRNA表达水平的影响

【作者】 黄梅；

【导师】 刘福友；

【作者基本信息】 成都中医药大学 ， 中西医结合临床， 2007， 硕士

【摘要】 目的通过观察三七二醇预处理对局灶性脑缺血大鼠的神经功能、病理学改变,Nissl体变化、脑梗死体积、脑组织中Glibal fibrillary acidic protein(GFAP)、interleukin-1β(IL-1β)mRNA表达水平的影响,探讨三七二醇预处理能否诱导缺血耐受及可能的机制。方法SD大鼠随机分为空白组、模型组(MCAO)、预缺血+模型组(IPC+MCAO)、三七二醇预处理组(PDS+MCAO)、三七二醇治疗组(MCAO+PDS)、假手术组(SS+SS)6组。空白组不给予任何干预;MCAO组连续3d腹腔注射生理盐水后,给予2h MCAO,再灌注22h后处死动物;IPC给予10min IPC,连续腹腔注射生理盐水3d后再给予2h MCAO,再灌注22h后处死;SS组给予10min SS,3d后给予2h SS,24h后处死动物;PDS+MCAO组给予3d PDS后给予2h MCAO,造模后24h处死;MCAO+PDS组予2h MCAO,再予PDS治疗3d后处死。造模后观察大鼠神经功能缺失情况,HE染色观察病理改变、Nissl染色观察Nissl体、TIC染色检测梗死体积、免疫组化染色观察GFAP阳性表达、RT-PCR检测IL-1βmRNA表达水平。实验数据用SPSS13.0处理,统计用ONE-WAY ANOVA检验。结果:IPC+MCAO、PDS+MCAO组脑梗死体积表达较MCAO组减少,三组间有统计学差异(p＜0.05)。IPC+MCAO、PDS+MCAO组的尼氏体积分光密度、GFAP表达水平较与MCAO组比较升高,三者间有统计学差异(p＜0.05)。IPC+MCAO、PDS+MCAO组的IL-1βmRNA表达水平与MCAO组比较降低,三者间有统计学差异(p＜0.05)。结论:PDS预处理诱导了脑缺血耐受,对将要发生的严重脑缺血具有保护作用。星形胶质细胞的活化及抑制炎性反应可能是三七二醇预处理诱导脑缺血耐受重要机制。更多还原

【Abstract】 Objective:To investigate the influence of Panasadiol Saponins(PDS) preconditioning on the cerebral ischemia in rats and the probable mechanisms,we set up a focal cerebral ischemia model and observe the changes of nerve function, Nissl body,infarct volume,the expression of Glial fibriUary acidic protein(GFAP) and interleukin-1β(IL-1β)mRNA in brain after acute cerebral ischemia.Method:SD rats randomly divided into six groups:blank,model(NS+ MCAO),ischemic preconditioning(IPC+MCAO),PDS preconditioning(PDS+M CAO),PDS treatment(MCAO+PDS),sham operated(SS+SS).Nonintervention to blank group;after intraperitoneal injection of NS continuously 3 days to mode 1 group,gave 2h MCAO,executed after reperfusion 22h;10 min IPC to IPC group,3 days later,gave 2h MCAO,executed after reperfusion 22h;10min SS to SS group,gave 2h SS after 3 days,executed after 24h;PDS for 3 days t o PDS+MCAO group,gave 2h MCAO,executed after model building 24h.2h MCAO to MCAO+PDS group,executed after PDS treatment for 3 days;obser ving the information of loss of nerve function of rats after model building,pat hological changes of HE dyeing,Nissl body observation of Nissl dyeing,inf arct volume detection of TTC dyeing,observation of GFAP masculine expressi on of immunity tissue chemistry dyeing,detection of IL-1βmRNA express lev el of RT-PCR.Experimental data was dealt with SPSS13.0,tested statistic with ONE-WAY ANOVA.Result:cerebral infarction volume of IPC+MCAO and PDS+MCAO group are more decreased than MCAO group,there are statistic differences among the three groups(p＜0.05),the IOD of nissl body and GFAP express levels of IPC+MCAO and PDS+MCAO group are higher than MCAO group,there are statistic differences among the three groups(p＜0.05).IL-1βmRNA express levels of IPC+MCAO and PDS+MCAO group are decreased compare with MCAO group,there are statistic differences among the three groups(p＜0.05). Conclusion:PDS preconditioning may intluce ischemic toleranee in organism, and protects against neuronal damages after subsequent lethal ischemic insults.The activation of Astrocytes and inhibit inflammatory reaction are possibly the important mechanism of PDS preconditioning induced ischemic tolerance.更多还原