【作者】 李国栋；

【导师】 王长海；

【作者基本信息】 烟台大学 ， 生物化工， 2008， 硕士

【摘要】 为探讨海洋微藻对某些重金属离子的生物吸附特性,以球等鞭金藻及Cd2+、Cu2+、Zn2+、Cr6+等四种重金属离子为材料,研究了四种重金属离子对球等鞭金藻生长及生化成分的影响,以及不同浓度金属离子条件下,氮和磷营养盐对球等鞭金藻生长及生化成分含量的影响;同时,探讨了球等鞭金藻对四种重金属离子的吸附特性及其对重金属离子的吸附动力学;获得以下主要结果:1、低浓度的重金属离子对球等鞭金藻的生长无明显抑制作用,在Zn2+<4.0 mg/L,Cd2+<5.0 mg/L,Cu2+<3.0 mg/L和Cr6+<10.0 mg/L的条件下,球等鞭金藻的生长基本不受影响。随着离子浓度的升高,球等鞭金藻的细胞生物量、胞内叶绿素a含量及蛋白质含量随之逐渐降低,但多糖含量则是先略有升高然后下降。2、当培养基中Cu2+、Cd2+、Zn2+和Cr6+浓度分别为0.1 mg/L、0.005 mg/L、0.1mg/L和1.0 mg/L时,通过均匀实验,各种重金属离子浓度下的最适生长氮浓度分别为12.69、14.93、15.94、12.26 mg/L,最适生长磷浓度分别为1.13、1.21、1.33和0.85 mg/L。3、根据我们的实验结果,球等鞭金藻细胞对Zn2+、Cd2+、Cr6+和Cu2+的生物吸附过程可以被分为快速吸附和缓慢吸附两个阶段;其对四种重金属离子的吸附能力顺序为:Cu2+>Cr6+>Zn2+>Cd2+。分别用Langmuir和Frenundlich模型对四种重金属离子的生物吸附过程进行了拟合,发现Frenundlich模型适合于球等鞭金藻对四种重金属离子的吸附,而对于Langmuir模型只有对Cu2+拟合的较好。更多还原

【Abstract】 To describe the characteristic of microalgae as the material for biosorbtion, a kind of marine microalgae, Isochrysis galbana, and Cd2+、Cu2+、Zn2+ and Cr6+ were chosen as the experimental material. The effects of the four heavy-metals of Cd2+、Cu2+、Zn2+ and Cr6+ on the growth of Isochrysis galbana and the concentration of some biological compounds in the cells were researhed. The effects of N and P on the growth of Isochrysis galbana under the different concentrations of the four heavy metal were also studied. And the adsorption characteristic, absorbtion kinetics and absorbtion isotherm of Isochrysis galbana on the four heavy-metals were discribed. The results were showed as follow.1. Under the lower concentration, the growth of the Isochrysis galbana would not be obstructed by the four heavy metals, especially when the concentration of Zn2+, Cd2+, Cu2+ and Cr6+ were lower than 4.0, 5.0, 3.0 and 10.0 mg/L. The biomass, chlorophyll-a and protein in the cells of Isochrysis galbana decreased with the increasing of the ion concentration, but, the content of polysaccharide in the cells increased with increasing ion concentration first, then decrease slowly.2. Under the concentration of Cu2+, Cd2+, Zn2+ and Cr6+ was 0.1mg/L, 0.005mg/L, 0.1mg/L and 1.0mg/L, the optimum concentrations of N and P in culture medium were determined by the method of uniform design, which were 12.69, 14.93, 15.94 and 12.26 mg/L for N, and 1.13, 1.21, 1.33 and 0.85 mg/L for P, respectinely.3. According to our data, the biosorption process of the cells of Isochrysis galbana for the four heavy metals could be divided the fast and the slow stages. The biosorption efficiency of the cells of Isochrysis galbana to the four heavy metals was Cu2+> Cr6+ >Zn2+ >Cd2+. Langmuir and Freundilich isotherm could be used to describe the sorption processes,the results showed that the Frenundlich model was in good agreement with the biosorption of Isochrysis galbana for Zn2+, Cd2+, Cr6+ and Cu2+, while the Langmuir isotherm model was only fitted for Cu2+.更多还原