【作者】 袁文菊；

【导师】 肖传斌； 刘为民；

【作者基本信息】 河南农业大学 ， 基础兽医学， 2008， 硕士

【摘要】 本试验研究以粤禽黄鸡、成年鸽、仙湖白鸭为试验材料,采用HRP逆行追踪法,将50% HRP溶液分别注射青年鸡小脑Ⅳ、Ⅴ、Ⅵ、Ⅶ、Ⅷ、Ⅸ各叶,成年鸡、鸽、鸭同时注射小脑Ⅵ、Ⅶ、Ⅷ三叶,逆行追踪直接投射于禽类小脑各叶的起始神经元。对端脑及间脑、脑干及小脑进行冰冻切片,TMB呈色,观察脑内标记细胞出现的位置。结果表明:(1)在青年鸡小脑分别注射Ⅳ、Ⅴ、Ⅵ、Ⅶ、Ⅷ、Ⅸ各叶,除第Ⅸ叶外,双侧端脑的海马结构的旁海马内侧区(APHm)出现大量标记细胞,海马(HP)、旁海马中间内侧区(APHim)、旁海马中间外侧区(APHil)、旁海马外侧区(APHl)以及后背外侧区(CDL)均无标记细胞出现。标记细胞集中而密度稍大,在APHm分布范围长约5～11㎜,海马被标记区域约占海马总长度的40%以上。横断面观,见标记细胞分布于一个较小的区域,集中而密度稍大。细胞以球形为主,以接近脑室面一侧标记渐浅,细胞内酶标物不完全充满细胞,多在核周呈半月状分布,标记细胞直径大小为18.78±2.79μm。(2)在青年鸡小脑分别注射Ⅳ、Ⅴ、Ⅵ叶,在隔核偶尔出现少量逆标细胞,主要见于外侧隔核的背侧部,标记较疏淡。标记细胞体积较小,细胞直径平均大小约12.84±1.54μm。(3)在青年鸡随着注射点由Ⅳ叶到Ⅸ叶的后移,APHm出现标记细胞的可能性渐小。小脑前核群,包括延髓大细胞网状核、前庭降束核、下橄榄核、三叉下核、内外侧桥核、中脑的内侧螺旋核、被盖背侧核、豆状核等标记的可能性相应地增大。(4)在成年鸡将HRP同时注射于Ⅵ、Ⅶ、Ⅷ三叶,APHm和外侧隔核以及小脑前核群中的大细胞网状核、前庭降束核、下橄榄核、内外侧桥核、中脑的豆状核、内侧螺旋核等均出现标记细胞。(5)在鸽将HRP同时注射于Ⅵ、Ⅶ、Ⅷ三叶,在端脑内海马区无标记细胞出现,而外侧隔核有标记细胞出现,小脑前核群中的大细胞网状核、前庭降束核、下橄榄核、内外侧桥核、楔束外侧核、三叉神经脊束核、中脑的豆状核、内侧螺旋核等亦有标记细胞。(6)在鸭将HRP同时注射于Ⅵ、Ⅶ、Ⅷ三叶,在端脑内海马区和外侧隔核均无标记细胞出现,小脑前核群中的中脑的豆状核、内侧螺旋核等有标记细胞。用辣根过氧化物酶(HRP)逆行追踪法对直接投射于禽类小脑各叶的起始神经元进行定位研究,研究结果表明,在端脑,鸡存在海马向小脑的直接投射,这种投射主要终止于小脑的前叶、中叶;鸡和鸽存在隔核向小脑的直接投射。此外,在小脑前核群,对于几种实验动物均不同程度地有向小脑的直接投射,结果与前人对哺乳动物的研究相似。本研究结果还表明,在成年鸽和鸭端脑不存在海马向小脑的直接投射,关于该问题还有待于进一步深入研究。更多还原

【Abstract】 To determine the existence and the topographic territories of direct hippo-cerebellar projections in chicken.Solution of 50% HRP(horseradish peroxidase ) were injected into the foliaⅣthroughⅨof the cerebellum in youth chickens, and injected into the foliaⅥ,ⅦandⅧof the cerebellum in adult chickens and pigeons and ducks,respectively, for retrograde tracing the origins of the cerebrum projection to the cerebellum. Carried out the frozen sections for cerebrum and brain stem and cerebellum,stained with TMB,we observed the location of the labelled cells. The results indicated that:(1)A lot of labeled cells were observed in the parahippocampal area(APHm) of the bilateral hippocampal formation after injected in foliumⅣ～Ⅸexcept foliumⅨin youth chickens. In other areas the labeled cells weren’t observed.The density of labeled cells was centered and large,the length was 5～12㎜.The labeled area accounted more than 40% of the length.The cells in the dorsomedial hippocampus were labeled heavily and their average size was 18.78±2.79μm。(2)In addition, afterⅣthroughⅥfolium injection small number of labelled cells were also found in the lateral septal nucleus .The cells in the in the septal nucleus were labeled lightly and their average size was 12.84±1.54μm.(3)With the injection site shifted from foliumⅣtoⅨ, the possibility of the APHm being labeled gradually declined ,but that of the pre-cerebellar nuclei ,including Nuc. magnocellular reticular, Nuc. parvocellular reticular , Nuc.vestibularis descendens, Nuc. inferior olivary, Nuc. inferiorin trigeminal, Nuc.pontine and Nuc. spiriformis medialis increased respectively.(4)We observed the labelled cells in APHm, Nuc. septal, Nuc. magnocellular reticular, Nuc. parvocellular reticular , Nuc.vestibularis descendens, Nuc. inferior olivary, Nuc. inferiorin trigeminal, Nuc.pontine and Nuc. spiriformis medialis after injected from foliumⅥtoⅧof adult chickens.(5)We observed the labelled cells in Nuc. septal, Nuc. magnocellular reticular, Nuc. parvocellular reticular , Nuc.vestibularis descendens, Nuc. inferior olivary, Nuc. inferiorin trigeminal, Nuc.pontine, Nuc. cuneate lateral , Nuc. spinal of trigeminal and Nuc. spiriformis medialis after injected from foliumⅥtoⅧof pigeons,but didn’t found labeled cells in hippocampus.(6)We observed the labelled cells in Nuc. lentiform and Nuc. spiriformis medialis after injected from foliumⅥtoⅧof ducks.But didn’t found labeled cells in hippocampus and Nuc. septal.In a word,to determine the existence and the topographic territories of direct hippo-cerebellar projections in poultry. These results indicated that there are direct projections from the hippocampal formation to the rostral and intermedial folia of the cerebellum in chicken and aren’t in pigeon and duck.As in mammal,the septal nucleus have tight connections with hippocampal formation in chicken.About this question will be further studied.更多还原