【作者】 魏钧；

【导师】 孙忠人；

【作者基本信息】 黑龙江中医药大学 ， 针灸推拿学， 2008， 硕士

【摘要】 目的:1.观察心痹贴贴敷内关穴对家兔心肌缺血的影响。2.通过研究心痹贴贴敷内关穴抗家兔心肌缺血的作用,初步探讨其可能的神经生理机制。方法:1.实验1部分:家兔40只,随机分成5组分别为空白组、模型组、安慰剂组、硝酸甘油组、心痹贴组。每组均分别应用垂体后叶素和异丙肾上腺素造成急性心肌缺血模型和亚急性心肌缺血模型,通过光镜、多导生理记录仪、生物化学和免疫组化等方法,从组织形态学、生理学、生物化学、免疫组化等不同层面观察心痹贴贴敷内关穴对家兔心肌缺血的影响。2.实验2部分:家兔24只,随机分成3组,在下丘脑室旁核被损毁模型条件下,应用垂体后叶素造成急性心肌缺血模型,观察心痹贴贴敷内关穴抗心肌缺血的作用并探讨其神经生理机制。结果:1.与其他组相比较,心痹贴组心电图的恢复时间明显缩短(p＜0.05);光镜显示心痹贴组缺血心肌组织结构损伤轻,其总体作用优于阳性对照组。2.心痹贴组血清SOD含量均明显高于其他组(p＜0.05):血清MDA含量均明显低于其他组(p＜0.05):且心痹贴组增加SOD活性、抑制MDA增高方面优于硝酸甘油组(p＜0.05)。3.心痹贴能下调家兔缺血心肌组织Caspase-3基因表达,心痹贴组的Caspase-3表达弱于其他组(p＜0.05)。4、心痹贴组与PVN毁损组心电图ST段偏移均低于模型组,有显著差异(p＜0.05);心痹贴治疗组心电图ST段偏移低于PVN毁损组,有显著差异(p＜0.05)。结论:1.心痹贴贴敷内关穴可缩短急性心肌缺血家兔心电图恢复正常的时间。2.心痹贴贴敷内关穴可使减少家兔缺血心肌的氧自由基损伤,增强其抗氧化能力。3.心痹贴能够通过抑制和阻断心肌细胞过度凋亡的发生,拮抗儿茶酚胺类药物对心肌的毒性作用而起到保护心肌的作用。4.下丘脑室旁核有可能是心痹贴抗家兔急性心肌缺血的中枢神经生理传导的核团之一。更多还原

【Abstract】 Objective:To observe the effect of Xinbitie transdermal patch acupoint application on anti-myocardial ischemia and investigate it’s neural mechanisms on rabbit models.Methods:1.experiment partⅠ,40 rabbits was divided into 5 groups randomly, applied pituitrin or isoproterenol respectively causing the acute or subacute myocardial ischemia models.Light microscope,electron microscope, guide polysiological recorder,biochemistry,in situ end-labeling, immunohistochemistry and so on methods and in the cellular molecular level to discuss the combination mechanism of rabbits’ myocardial damage on the process of occurrence,developments,and changes,from which get to know the effect and mechanism of Xinbitie transdermal patch acupoint application for anti-myocardial ischemia comparatively and deeply.2.experimentⅡ,24 rabbits divided into 3 groups randomly.To observe the neurophysiologic mechanism of Xinbitie transdermal patch on the acupoints for anti-myocardial ischemia under the condition of destruction on the nucleus of hypothalamus models,the pituitrin was used to prepare the acute myocardial ischemia model.Result:1.Compared with the other groups Xinbitie transdermal patch group could shorten the recovery time of electrocardiogram and had significant difference(p＜0.05).2.Xinbitie transdermal patch group could reduce MDA and elevate SOD; the difference between Xinbitie transdermal patch group and nitroglycerin group was obvious(p＜0.05).3.Caspase-3 gene expression of Xinbitie transdermal patch group was lower than the other groups(p＜0.05).4.The excursion of ST potential ECG in Xinbitie transdermal patch group and impaired PVN group were lower than model group(p＜0.05);the difference between Xinbitie transdermal patch group and impaired PVN group was obvious.(p＜0.05)Conclusion:1.Xinbitie transdermal patch on the acupoints can improve the electrocardiogram appearance;diminish the damage of oxygen free radical, strengthen the ability of the rabbit on anti-oxidation;restrain and block the excess apoptosis of myocardial cell.2.PVN maybe one of central nerve nuclear mission which involve the process of Xinbitie transdermal patch acupoint application on anti-myocardial ischemia.更多还原