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PTEN和C-myc在肺癌中的表达及意义
The Expression of C-myc, PTEN in Lung Cancer and Its Significance
【作者】 钟甜；
【导师】 梅同华；
【作者基本信息】 重庆医科大学 ， 内科学， 2008， 硕士
【摘要】 目的:探讨抑癌基因PTEN和癌基因C-myc在肺癌发生、发展中的作用及其相关性。方法:采用免疫组织化学链霉菌抗生物素蛋白-过氧化酶法(S-P法),检测肺癌和肺正常组织切片中PTEN和C-myc蛋白表达。结果:(1)C-myc蛋白在肺癌组织中的阳性表达率高于肺正常组织,具有显著性差异(P<0.01);而PTEN蛋白在肺癌组织中的阳性表达率显著低于肺正常组织(P<0.01)。(2)①PTEN和C-myc在不同病理类型、组织分化和有无淋巴结转移组之间的差异无显著性(P>0.05);②从中-高分化的肺癌组织到低分化肺癌组织C-myc表达有增强的趋势;相反,PTEN表达有降低的趋势;③C-myc在有转移组的肺癌组织中阳性表达率高于无转移组;相反,PTEN在有转移组的肺癌组织中阳性表达率低于无转移组,但差别都无显著意义(P?0.05)。(3)C-myc和PTEN在肺癌组织中的表达呈负相关(r=-0.4479,P< 0.01)。结论:癌基因C-myc蛋白过度表达、抑癌基因PTEN蛋白的表达缺失在肺癌的发生、发展及转移过程中起促进作用,有望成为肺癌诊断和预后判断的标记物。
【Abstract】 Objective : To investigate the correlation between the expressions of tumour suppressor gene PTEN and oncogene C-myc in lung carcinoma.Methods : The expressions of PTEN and C-myc protein in normal lung tissue and lung carcinoma were observed by S-P immunohistochemical assay.Results : (1)The expression of C-myc in the lung carcinoma tissues was significantly higher than that in the normal lung tissues(P<0.01); The positive expression of PTEN protein in the lung carcinoma tissues was significantly lower than that in the normal lung tissues(P<0.01).(2)①Difference was not found in groups of pathological types , differentiated degree and lymph node metastasis (P>0.05);②The expression of C-myc might increase concomitant with the progress from well group lung cancer to poor group lung cancer.On the contrary,a general decreasing tendency of PTEN seemed to accompany;③The positive rate of C-myc was higher in lymph node metastasis than that in non-lymph node metastasis,though the positive rate of PTEN was lower in lymph node metastasis than that in non-lymph node metastasis. (3) The level of C-myc protein expression was negatively correlated with PTEN expression(r=-0.4479,P< 0.01).Conclusion: C-myc protein overexpression and the underexpression of PTEN was closely related to clinicopathological features in lung carcinoma,and could be used as a marker of tumour burden of lung carcinoma.