【作者】 邓亚玲；

【导师】 鲁建华； 张文芳；

【作者基本信息】 兰州大学 ， 眼科学， 2008， 硕士

【摘要】 目的研究小窝蛋白-1（CAV-1）mRNA在正常及紫外线照射的白内障模型大鼠晶状体上的表达,探讨其在氧化性白内障发生过程中的作用。方法取50只大鼠的晶状体于MEM培养液中培养8h后随机分为A组（对照组25只）B组（UV照射组25只）。实验组:紫外线照射晶状体30min,加入MEM培养液,于37℃下,置5%CO₂培养箱中培养,分别在加入培养液后0、6、12、24及48 h时,应用荧光realtime RT-PCR技术检测晶状体中小窝蛋白-1的表达量,对照组不照射,与同等条件下用同样的方法检测小窝蛋白-1的表达量。结果紫外线照射30min后,采用RT-PCR技术测小窝蛋白-1mRNA表达水平随着时间的延长有逐渐下降的趋势。0h:实验组（0.5920±0.1254）对照组（1.0242±0.1117）（P＜0.05）两组相比差异有显著意义;6h:实验组（0.3914±0.2070）对照组（0.9662±0.1286）（P＜0.01）两组相比差异有显著意义;12h:实验组（0.2658±0.0938）对照组（0.9248±0.1545）（P＜0.01）两组相比差异有显著意义;24h:实验组（0.1244±0.0953）对照组（0.8840±0.0710）（P＜0.01）两组相比差异有显著意义;48h:实验组（0.0598±0.0414）对照组（0.8420±0.1023）（P＜0.01）两组相比差异有显著意义。结论紫外线照射对鼠晶状体上皮细胞产生影响,并导致细胞小窝蛋白-1表达量减少,小窝蛋白-1的减少在氧化性白内障的发生中有一定作用。推测小窝蛋白-1对维持氧化损伤时晶状体透明具有保护意义。更多还原

【Abstract】 Objective: To study the expression of caveolin-1 mRNA in lens of normal rats and rats radiation by ultraviolet radiation. In order to explore its role in development of oxidative cataract.Methods 50 rat lens in Minimum Essential Media in the culture medium after 8 hours were divided into Group A （control group 25） and Group B （UV-irradiation 25）. Experimental group: ultraviolet radiation lens 30 min after joining Minimum Essential Media medium at 37℃, 5% CO₂ incubator home in the culture, To detect the expression of caveolin-1 using fluorescence realtime RT -PCR in the control medium by adding 0, 6, 12, 24 and 48 hours. The methed and condition in control group were as same as the experimental group,but no ultraviolet radiation .Results After ultraviolet irradiation 30 min, and by RT-PCR to determine, the caveolin-1 mRNA expression level had been a downward trend;with time extension after ultraviolet irradiation 30 min by RT-PCR ;0 hours: the experimental group （0.5920±0.1254） were different from control group （1.0242±0.1117） （P <0.05） significantly; 6 hours: the experimental group （0.3914±0.2070） were different from control group （0.9662±0.1286） （P <0.01） significantly; 12 hours: the experimental group （0.2658±0.0938） were different from control group （0.9248±0.1545） （P <0.01 ） significantly; 24 hours: the experimental group （0.1244±0.0953） were different from control group （0.8840±0.0710） （P <0.01） significantly; 48 hours: the experimental group （0.0598±0.0414） were different from control （0.8420±0.1023） （P <0.01） significantly.Conclusions The rats lens lens epithelial cells were effected by ultraviolet irradiation lead to the expression reduction of caveolin-1. The expression reduction played a role in the incidence of cataract. We can speculate that caveolin-1 played a protective role in maintening lens transparent of oxidative damage.更多还原