【作者】 刘书文；

【导师】 张有成；

【作者基本信息】 兰州大学 ， 外科学， 2008， 硕士

【摘要】 目的:探讨缺血后处理对大鼠肝脏缺血再灌注损伤的保护作用和eNOS表达的影响。方法:建立大鼠肝脏局部缺血再灌注(IR)模型,将75只健康雄性SD大鼠随机分为假手术组、缺血再灌注组及缺血后处理组,缺血后处理组于缺血60min恢复血液再灌注前,给予六个循环的灌注10s/阻断10s的缺血后处理,观察血清天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、肝组织中丙二醛(MDA)、超氧化物歧化酶(SOD)的含量变化,并行肝组织电镜、病理学检查及免疫组化法检测肝细胞eNOS蛋白的表达。结果:与缺血再灌注组相比,缺血后处理组肝酶的漏出及肝组织MDA明显降低(P＜0.01),而SOD含量则显著升高(P＜0.01),同时肝组织病理形态学损伤亦明显减轻,缺血7min后eNOS蛋白表达更明显(P＜0.01)。结论:缺血后处理可通过抑止再灌注后氧自由基的过量生成、促进抗氧化剂的生成及通过激活再灌注损伤补救激酶(RISK)通路,减轻肝细胞损伤,发挥保护效应。更多还原

【Abstract】 Objective:To investigate the protective effect of ischemic postconditioning (IPo)on hepatic ischemia-reperfusion injury and the expressions of eNOS in rats.Methods:With a rat model of acute liver ischemia-reperfusion,75 healthy male Sprague-Dawley rats were randomly divided into shamoperated(S), ischemia-reperfusion(IR)and IPo group.Before persistent reperfusion,animals were given six brief reperfusion-ischemia in IPo group.The sample of blood and hepatic tissue of all groups were taken after experiment.Results:Compared with IR group,the concentrations of both aspartate aminotransferase(AST)and alanine aminotransferase(ALT),the concentrations of malondialdehyde(MDA)in hepatic tissue in IPo group decreased markedly(P＜0.01), while the concentrations of superoxide dismutase(SOD)increased significantly(P＜0.01),the hepatic congestion and pathological damage also relieved obviously.The expression of eNOS protein in Ipo group was significantly higher than the two remaining groups(p＜0.01).Conclusion:IPo can attenuate hepatic ischemia-reperfusion injury by activating the prosurvival kinases eNOS in accordance with the RISK pathway and reducing the concentrations of oxygen free radical.更多还原