【作者】 秦丽丽；

【导师】 哈斯巴根；

【作者基本信息】 内蒙古师范大学 ， 植物学， 2008， 硕士

【摘要】 运用RAPD方法,研究了蒙古韭(Allium mongolicum Regel)10个居群间和4个居群内不同亚居群间的遗传多样性水平。结果表明,蒙古韭不同10个居群间的遗传多样性指数为0.2977;居群间的遗传分化系数为0.0174,即1.74%的遗传变异来自于居群间,98.26%的遗传变异都来自于居群内部的不同个体间;不同居群间的基因流为28.235,这可能与蒙古韭较大的分布范围有关;蒙古韭10个居群间遗传距离与地理距离之间没有相关性。蒙古韭同一居群内不同亚居群间的遗传多样性指数平均为0.3447,表明该种依然保持着很高的遗传多样性水平;蒙古韭同一居群内不同亚居群间的基因流平均值为1.8650,这可能与其传粉方式和种子传播机制有关;蒙古韭同一居群内不同亚居群间的地理距离与遗传距离具有相关性。本研究所选择的4个居群当中,阿拉善左旗亚居群的遗传多样性水平最高,遗传多样性指数为0.2707,而赛汗塔拉亚居群的遗传多样性水平最低,其遗传多样性指数为0.2060,表明蒙古韭对干旱生境更具有适应能力及进化潜力。蒙古韭的遗传多样性水平较高,对环境变化的适应能力较强,是进化十分活跃的物种,应合理利用和保护。更多还原

【Abstract】 Based on ten populations of Allium mongolicum, this paper assesses the genetic diversity among all populations selected, and that among different sub-populations within one population, which only involves 4 of total ten populations. The method of CTAB is used to obtain the genome DNA which is amplified by RAPD. It indicates that the Shannon index of Allium mongolicum is 0.2977 among ten different populations and the genetic differentiation index is 0.0174 among different populations, that is, 1.74% genetic variation is among populations while 98.26% is within one population. The index of gene flow is 28.235 among different populations, which may relate to their more extensive distribution. There is no interrelation between genetic distance and geographic distance of different populations.Four populations of Allium mongolicum are selected for illustrating the genetic diversity among sub-populations of each population. It reveals that Shannon index is 0.3447 among sub-populations within one population, showing rich genetic diversity of Allium mongolicum. The index of gene flow is 1.8650 among different sub-populations within one population. It may relate to its mechanism of pollination and scattering seed. The genetic distance correlates to geographic distance among sub-populations of each population. Of the four populations, that of Left Alashan Banner population shows the richest genetic diversity, whose index among sub-populations of population, the highest, is 0.2707, while the index of population in Saihantala, the lowest, is 0.2060, showing the poorest genetic diversity of this population, which is seen as the proof of the species’ability to adapt an arid environment and its evolutionary potential.Allium mongolicum have a rich genetic diversity, it has strong evolutionary potential and large adaptability to the change of environment, as a result, it is necessary to make reasonable utilization and conservation.更多还原