【作者】 蒋进；

【导师】 依巴代提； 艾尼瓦尔；

【作者基本信息】 新疆医科大学 ， 药理学， 2007， 硕士

【摘要】 研究罗勒水提取物对血管内皮细胞（VEC）损伤的保护作用及其对血栓闭塞性脉管炎大鼠模型的治疗作用,进一步探讨罗勒水提取物抗血栓形成的部分作用机制。方法:分别采用皮下注射肾上腺素（Adr）法和月桂酸股动脉注射法建立血管内皮细胞损伤和血栓闭塞性脉管炎大鼠模型,以复方丹参片为阳性对照。胸主动脉切片检查VEC损伤大鼠血管内皮受损情况,分别采用放射免疫法、酶联免疫吸附测试法（ELISA）测定VEC损伤大鼠血浆内皮素、血管性假血友病因子和组织型纤溶酶原激活物含量;观察血栓闭塞性脉管炎大鼠的体征变化、血液流变学指标及对血浆血栓素B₂、6-酮-前列腺素F₁a含量和二者比值的影响。结果:罗勒水提物能显著减轻VEC损伤大鼠动脉内皮层的损伤程度,明显降低血浆内皮素、血管性假血友病因子水平,显著升高血浆组织型纤溶酶原激活物水平,与模型组相比有显著性差异;同时能明显改善血栓闭塞性脉管炎大鼠的血液流变学指标,明显降低血栓闭塞性脉管炎大鼠血浆血栓素B₂水平和血栓素B₂/6-酮-前列腺素F₁a比值,且作用强于复方丹参片组。结论:罗勒水提取物对受损血管内皮细胞具有良好的保护作用,对血栓闭塞性脉管炎大鼠模型具有较好的治疗作用,其机制可能与调节内皮细胞的分泌功能,降低血粘度、抗血小板聚积,维持血浆血栓素A₂和前列环素平衡等功能有关。更多还原

【Abstract】 Objective:To study the protective effect of aqueous extract of ocimum basilicum on endothelial cell injury inducing by adrenaline and the therapeutic effect of that on the rat models with thromboangitis obliterans.Methods:Models of endothelial injury and thromboangitis obliterans were respectively established via pro injecting adrenaline and injecting lauric acid into the femoral artery of rats.The damaged degree of vascular endothelial cell was evaluated by aorta pectoralis pathologic slice.The content of ET, vWF and t-PA in rat plasma was measured by Radioimmunoassay and ELISA;Observed the changes of physical signs,the indexes of hemorheology and the contents of TXB₂ and 6-Keto-PGF₁ a in plasma of thromboangitis obliterans rats following the treatment with aqueous extract of ocimum basilicum.Results:Compared to those of the model group,aqueous extract of ocimum basilicum can significantly lessen the degree of artery endothelial cell injury,significantly lower the content of ET and vWF in endothelial injury rat plasma and increase the content of t-PA.Meanwhile,significantly improved indexes of hemorhelolgy,decreased content of TXB2 and declined ration of T/K. Conclusion:Aqueous extract of ocimum basilicum presented the protective effect on vascular endothelial cell and significant therapeutic effect on experimental thromboangitis obliterans prepared among rats.The possible mechanisms for this effect may be due to improved the excrete function of vascular endothelial cell,improvement in hemorheology and enhanced anti-thrombus function of vessel endothelia.更多还原