【作者】 李平；

【导师】 傅先明；

【作者基本信息】 安徽医科大学 ， 外科学， 2008， 硕士

【摘要】 目的:探讨吗啡成瘾大鼠伏核毁损术后行为学改变与脑内单胺类神经递质变化的相关性以及毁损前后的脑影像学变化。方法:通过给大鼠每日两次腹腔注射吗啡,逐日递增吗啡用量,制作急性吗啡成瘾模型。使用立体定向技术毁损伏核,对比毁损前后吗啡成瘾大鼠的行为学变化;高效液相色谱—荧光检测法测定脑内不同结构的单胺类神经递质含量,比较毁损前后递质含量的变化。综合分析吗啡成瘾大鼠伏核毁损术后行为学改变与脑内单胺类神经递质变化的相关性。条件性位置偏爱(CPP)试验观察毁损术前后吗啡成瘾大鼠的行为学变化。毁损术前后行磁共振(MRI)影像学检查,观察影像学的变化。综合分析吗啡成瘾大鼠伏核毁损术后行为学改变与影像学变化,从而判断手术戒毒的疗效。结果:1.急性吗啡成瘾模型制模成功率较高,大鼠成瘾前后以及毁损术后行为学变化明显,毁损伏核后可缓解成瘾大鼠的戒断症状。2.毁损伏核后大鼠相关脑区多巴胺(DA)、去甲肾上腺素(NE)含量下降,5-羟色胺(5-HT)含量升高,毁损组较对照组的变化明显(P﹤0.01),差异有显著性。3.成瘾后大鼠对CPP箱的白盒有明显的位置性偏爱,大鼠成瘾前后以及毁损术后行为学变化明显,真毁损伏核与假毁损伏核对大鼠的行为改变不同,毁损伏核后可改善成瘾大鼠的戒断症状。4.毁损术前后影像学有明显变化:大鼠伏核毁损区脑组织水肿明显。结论:1.吗啡成瘾大鼠立体定向毁损伏核后相关脑区单胺递质含量的变化与毁损术前后行为学的改变有一定的相关性。2.吗啡成瘾大鼠立体定向毁损伏核后,戒断症状明显改善,影像学显示毁损术后大鼠伏核区脑组织水肿明显。立体定向毁损伏核手术准确有效。更多还原

【Abstract】 ObjectiveTo investigate the relationship between the level of monoamine neurotransmitters and the change of ethology after nucleus accumbence lesioned in morphine-addicted rats,and observe the imageological changes in brain regin of morphine-addicted rats before and after stereotaxic lesion.MethodsAn acute morphine-addicted rat mode was made with morphine by abdominal cavity injection. The interval of the injection is 12 hours. The dose of injection was added by daily from 5 mg/kg to 90 mg/kg within 10 days. Before and after stereotaxic lesion, the level of monoamine neurotransmitters in different brain regions was detected by a fluorescence detector (high performance liuid chromatography). The relationship between the monoamine neurotransmitters and ethological changes was analyzed after nucleus accumbence lesioned in the experiment rats.Results(1) There was a high successful rate in making acute morphine-addicted rats, and remarkable ethological changes in morphine-addicted rats before and after stereotaxic lesion.(2)There were significant difference in ethological changes between nucleus accumbens-lesioned group and nucleus accumbens pseudo-lesioned group.(3)After nucleus accumbence lesioned, the abstinence syndrome could lessen. The levels of DA, NE, 5-HT in the regions of prefrontal cortex, hippocampus and ventral tegmental area were obvious changed compared with the unlesioned group in morphine-addicted rats (P<0.01).(4) Before and after lesion, the imageology of the morphine-addicted rats’brain are different too, the brain edma of the nucleus accumbence region were evidently.Conclusions(1)There may have some relationships between changes of ethology and the levels of monoamine neurotransmitters in the morphine-addicted rat.(2)Before and after lesion, the imageology of the morphine-addicted rats’brain are different too,the brain edma of the nucleus accumbence region were evidently.更多还原