【作者】 曾明；

【导师】 董扬；

【作者基本信息】 上海交通大学 ， 外科学， 2007， 硕士

【摘要】 目的:了解硫酸钙人工骨复合MTX在Tris缓冲生理盐水的体外模拟环境中的释药特性。方法:以硫酸钙人工骨OSTEOSET?为载体,制备载药量20mg,质量百分比为4﹪的药粒。采用浸出法,以Tris缓冲生理盐水作为模拟体液,在37℃,转速50rpn的条件下,收集最初24小时内和后续20天的液体标本,经高效液相色谱分析药物浓度。结果:25粒药粒进入实验,20粒药粒完成全部实验设计。24小时内平均药物浓度初期为10.535mg/L,后期在4～6mg/L之间,累计药物浓度与时间平方根成指数关系。药物释放以4～6﹪的速度,持续稳定释放达18天。早期释药量较高,为10～11﹪。结论:OSTEOSET?硫酸钙人工骨复合MTX药物释放稳定,是良好的药物缓释载体。更多还原

【Abstract】 Objective: To investigation the character of drug release of calcium sulfate combined with MTX in the circumstance of Tris-buffered natural saline. Methods: The drug particles combined with 20 mg MTX, 4% in weight percentage, were made manually, as the calcium sulfate (OSTEOSET?) for the carrier. The experiment was carried under the condition of 37℃in temperature, rotation rate 50rpn. The mimesis of body fluid was Tris-buffered natural saline. The extraction method was adopted. Samples were collected every 2 hours in the first 24hours and everyday in the next 20 days. The drug concentration of each sample was analyzed through high performance liquid chromatogram. Results: Twenty-five particles were put into the experiment, and twenty were observed throughout the experiment. The average drug concentration of the first two hours was 10.535mg/L, the highest of the first twenty-four hours, and maintains between 4 mg/L and 6mg/L latterly. There was an exponential relation between the accumulative drug concentration and the square root of time. The drug release maintains stably for 18 days, under the rate of 4～6﹪. In the early period, the drug release rate was the highest, from 10 to 11 percent.Conclusion: The drug particles of calcium sulfate (OSTEOSET?) combined with MTX release stably, and the calcium sulfate (OSTEOSET?) was a better carrier of drug implants.更多还原