【作者】 蔡志凯；

【导师】 吴树清；

【作者基本信息】 内蒙古农业大学 ， 临床兽医学， 2007， 硕士

【摘要】 本实验以处于围产期的57头奶牛为研究对象,通过实验室检查结合临床症状查出其中健康的经产牛、健康的初产牛及患酮病、患脂肪肝、患胎衣不下的自然病例,在奶牛产前15天,产前8天,产前1天,产后1天,产后8天,产后15天和产后22天分别采颈静脉血,检测血清中甲状腺激素(T4和T3)、促甲状腺激素、碘、硒和谷胱甘肽过氧化物酶的含量、摸索出其动态变化并作了统计分析。实验结果显示:1.健康的经产牛围产期血清中T4、T3、TSH、I、Se、GSH-Px均呈现波浪式动态变化,最高值分别出现在产前1天,产后22天,产后1天,产前15天,产后22天,产后15天;TSH最低值出现在产前15天,其它检测指标最低值出现在产后1天。2.患酮病的奶牛围产期处于缺碘、缺硒状态,其产后甲状腺机能出现减退。3.患脂肪肝的奶牛围产期处于缺硒碘状态,患脂肪肝期间同时伴发甲状腺功能减退症。4.患胎衣不下的奶牛围产期硒碘含量低于正常值,其生产前后伴发亚临床甲状腺功能减退症。5.健康的初产牛围产期血清中T4、T3、TSH、I、Se、GSH-Px的动态变化趋势同健康的经产牛类似,总体上血清中T4、T3、I、Se、GSH-Px的含量值略低于健康的经产牛。6.本实验数据统计结果显示:奶牛血清中T4与TSH、T3、I、Se、GSH-Px的相关系数分别为-0.749,0.535, 0.691,0.655,0.603;T3与TSH、I、Se、GSH-Px的相关系数分别为-0.502,0.457,0.572,0.401;Se与GSH-Px的相关系数为0.820。更多还原

【Abstract】 57 cows in the perinatal period were used in the experiment.Through clinical symptom and laboratory examination, they were divided into five groups that were healthy multiparity cows,healthy primiparity cows,cows with fatty liver disease, cows with ketosis and cows with rtention fetal membrance. We experimented blood of antepartum 15,8,1 day and postnatal 1,8,15,22 day,then assayed Thyroid hormone, thyroid-stimulating hormone, Iodine ,Selenium, GSH-Px in blood-serum. At last,we analysed data by statistical way .The results showed that:1. Thyroid hormone, thyroid-stimulating hormone,Iodine,Selenium and GSH-Px of blood-serum in healthy multiparity cows presented wave change respectively during the Perinatal Period.Highest value was at antepartum 1day for T4, at postnatal 22days for T3,at postnatal 1 day for TSH,at antepartum 15 days for Iodine, at postnatal 22days for Se,at postnatal 15 days for GSH-Px.;lowest value at antepartum 15 days for TSH, at postnatal 1 day for others.2. Cows with ketosis showed Iodine deficiency disorders and Selenium deficiency disorders during the perinatal period, meanwhile hypothyroidism after childbirth .3. Cows with fatty liver disease showed Iodine deficiency disorders,Selenium deficiency disorders and hypothyroidism.4. The levels of thyroid hormone in serum samples of cows with rtention fetal membrance were significantly lower than that of comparison group (P<0.05),and the levels of Selenium and Iodine were significantly lower than normal value.5. Dynamic change curves of thyroid hormone,thyroid-stimulating hormone, Iodine ,Selenium and GSH-Px were similar between healthy multiparity cows and healthy primiparity cows. But most of numerical value of primiparity cows were lower than that of multiparity cows.6 .Statistical result from this study showed: Correlative coefficient of T4 vs TSH,T3,I,Se,GSH-Px respectively was -0.749,0.535,0.691,0.655,0.603; Correlative coefficient of T3 vs TSH,I,Se,GSH-Px respectively was -0.502,0.457,0.572,0.401;Correlative coefficient of Se vs GSH-Px was 0.820.更多还原