【作者】 吴彪；

【导师】 彭丹；

【作者基本信息】 中南大学 ， 外科学， 2007， 硕士

【摘要】 目的检测热休克蛋白70(HSP70)在骨巨细胞瘤中的表达，探讨其与骨巨细胞瘤Jaffe病理分级和Enneking外科分期的关系，给临床诊断治疗提供帮助。方法收集30例骨巨细胞瘤患者的手术标本，与10例骨软骨瘤进行对照，采用免疫组化S—P法检测HSP70在不同肿瘤中的表达情况，分析其与骨巨细胞瘤分期的关系。结果1．HSP70在骨巨细胞瘤组中表达高于骨软骨瘤组，二者有显著差异(P＜0.05)。2．按照Enneking外科分期：(1)．HSP70在骨巨细胞瘤1级组与骨软骨瘤组中的表达无差异性(P=1.000)，无统计学意义；(2)．HSP70在骨巨细胞瘤2级组和3级组中表达均显著高于骨软骨瘤组中表达(P＜0.05)；(3)．HSP70在骨巨细胞瘤2级组和3级组中表达均显著高于骨巨细胞瘤1级组(P＜0.05)；(4)．HSP70在骨巨细胞瘤2级组与骨巨细胞瘤3级组中表达差异性不显著(P＞0.05)。3．按照Jaffe分级，HSP70在骨巨细胞瘤3级组中表达与骨巨细胞瘤1级&2级组差异性不显著(P＞0.05)。结论1．HSP70在骨巨细胞瘤中表达显著高于骨软骨瘤，认为HSP70可能在骨巨细胞瘤发生发展中起作用。2．Enneking外科分期中，HSP70阳性表达率在骨巨细胞瘤2、3级显著高于骨巨细胞瘤1级，且差异有统计学意义，认为HSP70可作为判定骨巨细胞瘤侵袭性辅助指标。3．HSP70表达对于骨巨细胞瘤Jaffe病理分级无指导意义。更多还原

【Abstract】 OBJECTIVE In order to guide the treatment of GCT, this study is aimed to investigate the relation between the expression of HSP70 in GCT and classification (Jaffe & Enneking), by detecting expression of HSP70.METHODS 30 cases of GCT were collected including surgical operation spcimens,10 cases of osteochondroma were taken as control group. we detected the exprssion of HSP70 ,and analyzed the relation between the expression of HSP70 and classification(Jaffe & Enneking) of GCT.RESULTS1.The expression of HSP70 was significantly higher in GCT group than osteochondroma group,P<0.05.2.According to Enneking Grade: (1) .There was no statistical significance in the expression of HSP70 between GCT 1 group and osteochondroma group,P=1.000; (2) .The expression of HSP70 were significantly higher in both GCT 2 and GCT 3 group than osteochondroma group,P<0.05; (3) .The expression of HSP70 were significantly higher in both GCT 2 and GCT 3 group than GCT 1 group,P<0.05;(4). There was no significant difference in the expression of HSP70 between GCT 2 group and GCT 3 group,P>0.05.3.According to Jaffe Grade, There was no significant difference in the expression of HSP70 between GCT 3 group and GCT 1 & 2 group, P>0.05.CONCLUSION1.The expression of HSP70 in GCT is higher than osteochondroma , there is statistical significance ,HSP70 play a role in the occurence and develop course of GCT.2.On the premise of Enneking Grade,there is statistical significance between GCT 1 group and GCT 2, 3 groups in the expression of HSP70.The expression of HSP70 can indicated the invasion of giant cell tumor of bone.3.The expression of HSP70 is of no use for Jaffe Grade in GCT.更多还原