【作者】 佟欣；

【导师】 苏云明；

【作者基本信息】 黑龙江中医药大学 ， 药理学， 2007， 硕士

【摘要】 帕金森病(PD)是目前较为公认的一种难治的、缓慢进展的原因不明的脑病，尚没有十分满意的治疗方法。本文用熄风定颤胶囊灌胃给药的方法对PD模型大鼠进行了治疗，观察熄风定颤胶囊对PD模型大鼠行为学的影响；并测定熄风定颤胶囊对PD模型大鼠纹状体丙二醛(MDA)、超氧化物歧化酶(SOD)、谷光甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-PX)含量的影响；观测熄风定颤胶囊对PD模型大鼠黑质酪氨酸羟化酶(TH)的影响。本实验将6-羟基多巴胺(6-OHDA)注入大鼠纹状体制备PD模型大鼠，设计并成功地对PD模型大鼠进行了熄风定颤胶囊灌胃给药治疗，发现给药后PD模型大鼠的行为学有了显著改善(p＜0.01)。同时，纹状体丙二醛(MDA)含量下降，而谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-PX)含量升高，说明中药熄风定颤胶囊可以增强PD模型大鼠的抗氧化应激能力，从而减少氧化应激反应所致的DA能神经元损伤。组织形态学检测发现，酪氨酸羟化酶(TH)阳性细胞增加，证实了给予PD模型大鼠熄风定颤胶囊，增加了TH的阳性表达，从而减轻PD型大鼠的旋转行为。本实验不但证明了中药熄风定颤胶囊治疗PD的方法是可行的，而且是行之有效的；同时揭示了熄风定颤胶囊治疗PD模型大鼠的作用机制，从而为中药治疗PD奠定了理论与实验基础，为PD的治疗提供了一种新的思路和方法。更多还原

【Abstract】 It is accepted at present that Parkinson’s disease(PD) is an Idiopathic encephalopathy to be slowly progressive and intractable, and has no satisfying methods to treat yet.This paper describes and discusses a method to cure the PD-model white rat by giving the TCM of Xi-feng-ding-chan capsule and its influence to rat behavior, relevant bio-chemical criterion and histomorphology.In this experiment, author prepares a PD-model white rat by injecting the 6-OHDA into the rat’s caudate putamen(CPu), and succeeds in designing and giving the TCM of Xi-feng-ding-chan capsule to the rats. As a result, rat not only survival but also remarkable improvement(p＜0.01)in behavior after being given the TCM of Xi-feng-ding-chan capsule. At same time, MDA content decrease, the content of GSH and GSH-PX increase, so the TCM of Xi-feng-ding-chan capsule can improve anti-oxygenstress ability of PD-model white rat, and abate lethal damage to dopaminergic neurons bringing out by anti-oxygen stress.Histomorphology detection suggests that TH-positive cells increase after TCM treatment, and that demonstrate that TCM of Xi-feng-ding-chan capsule can increase the expression of TH-positive cells, and thereby inhibit the rat rotation behaviour.This experiment proves the TCM of Xi-feng-ding-chan capsule not only feasible but also valid; at one time, it make clear the function mechanism of this kind of TCM, and establishes a theoretical and experimental basis for curing PD by the TCM of Xi-feng-ding-chan capsule, and provides a new idea and method.更多还原