节点文献
椒目的化学成分研究
Studies on the Chemical Constituents from the Seeds of Zanthoxylom Bungeanum Maxim.
【作者】 王文泽;
【作者基本信息】 沈阳药科大学 , 药物化学, 2005, 硕士
【摘要】 本论文综合利用硅胶、聚酰胺、葡聚糖凝胶(Sephadex LH-20)柱色谱、制备薄层、重结晶等各种手段,对椒目95%乙醇提取物的化学成分进行系统分离,从中分离得到12个化合物,根据理化性质测定及波谱数据分析,鉴定了其中10个化合物的结构(见Table 2-1),分别为Obtucarbamate A(1)、表儿茶素(2)、槲皮素(3)、24-烯-环阿尔廷酮(4)、辛二酸(5)、硬脂酸(6)、β-谷甾醇(7)、胡萝卜苷(8)、异欧前胡素(9)、异茴芹素(10)。其中化合物1,2,3,4,5,9为首次从该属植物中分离得到。 本论文采用气相色谱法,首次对我国10个不同产地椒目药材中α-亚麻酸的含量进行了比较研究,通过方法学考察证实,精密度、重现性、稳定性良好,加样回收率等符合规定。结果表明:10个产地的样品中,河北、四川产椒目中α-亚麻酸含量较高。本文方法简便,结果可靠,可以用来评价和控制药材的质量,为合理开发利用椒目药用资源提供了参考依据。 本论文对椒目中具有明显抗哮喘作用的椒目油进行了药理活性研究,结果表明:椒目油具有明显的平喘、止咳、抗炎、抗过敏及抗应激作用,其作用机理可能与稳定肥大细胞膜以及减少AA脂氧酶代谢产物白三烯(LTB4、LTC4、LTD4、LTE4)的生成有关。
【Abstract】 Twelve compounds have been isolated from the seeds of Zanthoxylom bungeanum Maxim, by open-column, preparative TLC and Sephadex LH-20 column chromatography methods etc. According to their physico-chemical properties and spectroscopic analysis, ten compounds (see Table 2-1) have been identified as Obtucarbamate A (1), epicatechin (2), quercitrin (3), 9,19-cyclolanost-24-en-3-one (4), octanedioic acid (5), stearic acid (6), β-sitosterol (7), daucosterol (8), isoimperatorin (9), isopimpinellin (10). Compounds 1,2,3, 4, 5, 9 were isolated from the genus for the first time.The content of α-linolenic acid was determined in the seeds of Z bungeanum Maxim, from ten habitants in China. The contents of α-linolenic acid the seeds of Z bungeanum Maxim which obtained from Hebei and Sichuan were much more higher . The determination results indicated that the quality of the seeds of Z bungeanum Maxim, could be evaluated and controlled by GC. It was proved that the method was an effective and convenient reference standard for evaluating and controlling the herb of Z bungeanum Maxim.In addition, the results of the pharmacological research showed that the seeds oil of Z bungeanum Maxim. had the effects of anti- asthma, coughing stopping, anti-inflammatory and anti-stress. The mechanism was relative to stabilizing the membrane of mast cell to decrease the amount of LTB4, LTC4, LTD4 and LTE4, which were the metabolites of AA hydrolyzed by lipoxidase.
【Key words】 Seeds of Zanthoxylom bungeanum Maxim.; chemical constituents; coumarins; flavones;
- 【网络出版投稿人】 沈阳药科大学 【网络出版年期】2007年 01期
- 【分类号】R284
- 【下载频次】191