【作者】 刘鹏；

【导师】 吴爱国；

【作者基本信息】 第一军医大学 ， 普通外科学， 2006， 硕士

【摘要】 大肠癌是我国最常见的恶性肿瘤之一，其发病率有逐年上升的趋势。尽管近年来以手术为主的综合治疗使大肠癌患者的预后有很大改善，但术后5年生存率仍徘徊在50—60％左右。因此，深入研究控制大肠癌发生、发展过程的关键基因表达变化及其作用将为大肠癌早期诊断、预后判断和抗转移治疗提供理论依据。 目的：本研究旨在通过检测中期因子（Midkine，MK）mRNA、S期激酶相关蛋白2（S-phase kinase-associaced protein 2，Skp2）mRNA和Midkine蛋白、Skp2蛋白、MVD、p27^kip1蛋白在大肠不同病变组织的表达情况，探讨Midkine、Skp2在大肠癌中表达的临床意义及其与MVD、p27^kip1的关系，明确它们在大肠癌发生、发展中的作用，为临床诊断、治疗提供理论依据。 材料与方法：（1） 组织标本取自南方医科大学珠江医院2000．1-2004．10外科手术切除及结肠镜下活检标本。共选取83例大肠癌、18例大肠腺瘤和20例正常大肠黏膜标本。全部标本经4％多聚甲醛（含0.1％DEPC）固定液固定、常规脱水后石蜡包埋。（2） 应用原位杂交方法检测Midkine mRNA、Skp2 mRNA在大肠癌、大肠腺瘤和正常大肠黏膜组织中的表达。（3） 应用免疫组化SP更多还原

【Abstract】 Background: Colorectal cancer is one of the commonest malignant tumors in China and its morbidity is still increasing . Although the operation-based comprehensive therapies have greatly improved the prognosis of colorectal cancer patients in recent years, the postoperative 5-year survival rate, unfortunately, is still about 50%. Therefore, further exploring the alterations of key genes related to development and progression of CRC will be considered useful.Aims: In this study, We sought to investigate the expression of Midkine mRNA, Skp2 mRNA and Midkine protein, Skp2 protein, MVD, p27^kip1 protein in colorectal cancer, and to identify the clinical significance of Midkine, Skp2 and its relationship with the expression of p27^kip1 and MVD in colorectal cancer, to provide theoretical basis for early diagnosis and therapy of colorectal cancer.Materials and Methods: （1） Tissue specimens used for this study were obtained from 101 patients with colorectal cancer or adenoma, which were resected surgically or endoscopically at Hospital from Jan. 2000 to Oct. 2004. All the samples including 20 normal colorectal mucosae, 18 adenomas and 83 cancers were fixed in 4% Polyoxymethylene（0. 1%DEPC） and embedded in paraffin. （2） The expression of Midkine mRNA, Skp2 mRNA was detected by in situ hybridization and the更多还原