【作者】 刘旺根；

【导师】 吴景兰； 李建生；

【作者基本信息】 郑州大学 ， 人体解剖学与组织胚胎学， 2005， 硕士

【摘要】 目的:探讨黄芪建中汤对造模脾虚型慢性萎缩性胃炎(chronic atrophic gastritis,CAG)大鼠的治疗作用及机制,并为临床应用提供实验依据。 方法:将24只Wistar大鼠随机分为4组:1.正常(C)组:以相同条件喂养6只正常大鼠,作为正常对照组;2.CAG组:6只大鼠长期以2%的水杨酸钠灌胃并结合饥饱失常、劳倦过度的方法致成胃粘膜损伤;3.维酶素(Ⅴ)组:6只已造模大鼠使用维酶素治疗2ld;4.黄芪建中汤(RA)组:使用黄芪建中汤治疗6只已造模大鼠2ld。观察各组实验大鼠的一般状况、血常规、血液和胃液中生化指标、胃肠功能以及免疫功能的变化,运用原位杂交和细胞化学技术分别检测各组大鼠胃粘膜iNOS—mRNA、SDH、LDH、ANAE、ACP的表达量和糖原含量。 结果:与正常大鼠相比,CAG组大鼠一般状况欠佳,体重明显减更多还原

【Abstract】 Aim To study the mechanism for therapeutic effects of Radix Astragali on the established rat models of chronic atrophic gastritis(CAG) of spleen deficiency type and to provide the experimental data for clinic.Methods 24 Wistar rats were randomly divided into 4 groups: 1. Control (C) group, 6 normal rats fed under the same condition. 2. CAG group, 6 established rats models of chronic gastritis damaged by long-term oral administration of 2% saccylic acid combined with disorder administration and exhaustion. 3. Vitacoenzyme (V) group, 6 rats models were given vitacoenzyme tablets for 21d. 4. Radix Astragali (RA) group, 6 rats models were given Radix Astragali soup for 21d. The rats behavior, regular bio-markers of the blood sample and gastric juice, immunefunction and the expressions of iNOS - mRNA, SDH, LDH, ANAE, ACP and glycogen were examined by insitu hybridization and cytochemistry in each group respectively.Results When compared with the C group, the features/results were found in the CAG group as follows. 1. The rat body weight was reduced markedly with general weakness. 2. In the blood the content of hemoglobin, Fe, Zn, Mg, SOD, IgG and the clearance of carbon particles were decreased, while the ratio of Cu/Zn and MDA was increased. 3. In the gastric juice the pH value enhanced, the pepsin activity and the excretion of D-xylose declined. 4. The content of SIgA was decreased in the intestinal juice. 5. In the fine structure the gastric mucosae became atrophy andthinner with obvious erosion and more inflammatory cells. 6. The expressions of SDH. LDH, ANAE, ACP and glycogen were weaker, while the expression of iNOS gene was stronger. There were significant differences in all above parameters between the CAG group and C group, between the RA group and V group. P< 0.05.Conclusions The therapeutic effects of Radix Astragali predominated over Vitacoenzyme and could improve each symptom and sign of CAG almost recover to the normal level mainly through up-regulation of SDH, LDH, ANAE, ACP expressions and glycogen storage; down-regulation of iNOS expression and promotion of immune function.更多还原