【作者】 王东强；

【导师】 张国骏； 史丽萍；

【作者基本信息】 天津中医学院 ， 中医临床基础， 2005， 硕士

【摘要】 目的: 观察经方四逆散对肝损害的预防保护作用及机理研究。 方法: 采用石胆酸灌胃造成肝损害,检测血清丙氨酸氨基转移酶ALT、天门冬氨酸氨基转移酶AST、谷胱甘肽GSH、超氧化物歧化酶SOD、丙二醛MDA水平,观察肝组织病理学改变;用免疫组化的方法观察肝组织bax基因的表达情况。 结果: 经方四逆散能降低石胆酸灌胃造成肝损害小鼠血清ALT、AST、MDA升高的水平,能升高石胆酸灌胃造成肝损害小鼠血清SOD、GSH降低的水平;石胆酸灌胃能增强小鼠肝组织bax蛋白的阳性表达率,四逆散能降低灌胃石胆酸小鼠肝组织bax蛋白的阳性表达率。 结论: 经方四逆散能使肝损害小鼠肝组织病理变化程度明显减轻,并且四逆散药粉+米汤降AST、MDA明显,四逆散煎剂降ALT明显。四逆散能降低bax蛋白的表达,减少肝细胞的凋亡,保护肝组织。更多还原

【Abstract】 Objective:To observe the synteresis protection of Jingfang Sinisan on liver damage and the investigation of mechanism. Methods:Mice model of liver damage was established by pouring lithocholic acid. Check out tile serum level of alanine ammotransferase(ALT), asparti acid ammotransferase (AST), maleic dialdehyde(MDA), superoxide dismutase(SOD), glutathion(GSH), and observe the pathology variance of hepatic tissue, lithocholic acid can strengthen the rate of masccline expression of hepatic tissue; sinisan can depress the rate of masccline expression of hepatic tissue of mice by pouring lithocholic acid. Results:Sinisan obviously reduced the liver damage induced by lithocholic acid,decreased the plasma a concentration of ALT,AST and MDA,it also increased an decrease in SOD and GSH. Conclusion:Sinisan has remarkable effects on liver protection,it also shows that Sinisan used mitong obviously decrease AST,MDA and Sinisan Jianji obviously decrease ALT. the liver protection of Sinisan may come true through depress the expression of bax-protein and decrease hepatocellular apoptosis.更多还原