【作者】 王海燕；

【导师】 黄继轸；

【作者基本信息】 安徽农业大学 ， 茶学， 2004， 硕士

【摘要】 苦茶槭是槭树科槭属落叶小乔木或灌木，是我国广大林区一种有重要经济价值的野生药用植物资源，它既是良好的庭园和街道观赏树木，优质纤维树种、栲胶原料、黑色染料，又是具有治病健身之药效的新型保健茶饮料皋茶的原料。苦丁茶在我国已有2000年的历史，苦丁茶有很多的药理功效，近年来，苦丁茶以其独特的品质和保健功能作为一种非茶属天然植物饮料逐渐在国际、国内市场上走俏，在现代商品市场上崭露头角。本论文对皋茶中的活性成分及活性成分的抗癌、清除羟自由基作用作了初步研究，同时对苦丁茶的抗癌和清除羟自由基作用也作了初步的研究。 论文较为系统地研究了皋茶中的主要成分。在氨基酸的分析中，首次检测出游离氨基酸17 种，其中人体必需氨基酸7种；检测出蛋白水解氨基酸16种，其中人体必需氨基酸7种。 分别用乙醇、正丁醇和乙酸乙酯三种溶剂对皋茶中的酚类物质进行提取研究，发现，乙酸乙酯法较好。将此方法提取的粗提物纯化后作为标准物，制作工作曲线，测定了皋茶中酚类含量为20.06% ，同时就本实验条件确定了皋茶的定量方法，皋茶酚类的换算系数为2.5107。 对皋茶和苦丁茶的酚类物质作了紫外可见光谱分析，皋茶酚在280nm处有一很强的吸收峰，在480 nm处有一很弱的吸收峰。苦丁茶酚吸收光谱有三个吸收峰，最大吸收峰的波长为350nm，第二吸收峰的波长为270 nm，第三吸收峰的波长为267 nm。 本论文对皋茶和苦丁茶作了抗癌活性研究，研究表明纯化了的皋茶酚可能不具有抗癌活性，皋茶浸提物中除去酚类、生物碱类物质后其余的部分可能具有抗癌活性。苦丁茶浸提物中除去酚类物质后其余的部分也可能具有抗癌活性。皋茶、苦丁茶酚类以及茶多酚对羟自由基具有明显清除作用。在本实验设计的浓度范围内，皋茶酚类随着浓度的增加，清除率增大，两者呈线性相关，其相关系数达到0.9942，在浓度达到0.7 mg/ml时,其清除率约为100%；苦丁茶酚类浓度在0～0.2mg/ml之间,随着浓度的增加，清除率增大，在浓度为0.2 mg/ml时清除率达到最大，为35.05%，两者的相关系数为0.9985,从0.2mg/ml后随着酚类浓度的增加,清除率减小，呈下降趋势；茶多酚浓度在0～0.5mg/ml之间，随着茶<WP=6>多酚浓度的增加，清除率增大,在浓度为0.5 mg/ml时清除率达到最大，约为89.6%，相关系数为0.9953，但是0.5 mg/ml以后随着浓度的增加其清除率减小,呈下降趋势。更多还原

【Abstract】 Acer.ginnala Maxim, Aceraceae, Acer.L，which belongs to defoliation little arbor or bush,is a kind of wild medical plant resource with important economic worth in forest zone of our country. It is not only a good garden and street view and admire tree , with high-quality fibre seeds.Which is the raw materials of tannin extract and black dyestuffs，but also is material resourse of Gaocha, which is a new-type health care tea beverases with drug effect of curing the disease and building up body .Kudingcha has a history of 2000 years already in our country, having much pharmacobgy effiaency .In recent years,kudingcha has been recogrized its unique quality and health care function as a kind of natural plant beverage and selled well at world ,domestic market fradually ,showing up prominently on the modern commodity market. In this thesis, Gaochas, active compositions and its function of resistsing cancer and scavenging hydroxyl free radical were preliminary discussed. At the same time, kudingcha, its function resising cancer and scavenging hydroxyl free radical were also studied. The major active components in Gaocha were studied. In analysis of amino acid , for the first time ,we found 17 free amino acids in which there were 7 essencial amino acids, 16 hydrolyzed amino acids and 7 essential amino acids among them . Extracting phenols from Gaocha with different methods, and selected the best menthods.Distilling phenols with ethanol、n-butylalcohol and acetic ether ethyl acetate.The results showed the purified phenols was 93.14 percent with the menthod of acetic ether ethyl acetate.The purified phenols was 20.22 percent with the menthod of ethanol. The purified phenols was 41.28 percent with the menthod of n-butylalcohol. The standard curve was made with a purified phenols sample, and the content of phenols was examined to be 20.06 percent. Confirmed Gaocha, quantitative method under the experiment condition, and Gaocha phenols, conversion coefficient was 2.5017. Gaocha and kudingcha phenols were Studied with spectrum analysis within the range of 190-850nm. The result indicated Gaocha phenols have two peaks of absorption whithin the range. There was a very strong absorption peak in 280nm and a very weak absorption peak in 480 nm. Kudingcha phenols have three bigger absorptions. The maxium absorption warelength was 350nm, the medium absorption wavelength was 270nm, the minimum absorption wavelength is 267nm.In the thesis, anticancer activation of Gaocha and Kudingcha was preliminarily studied, the results indicate purified Gaocha phenols perhaps have no anticancer activation, while the part of removing phenols and alkloids may have anticancer activation. So is kudingcha, remainder of removing phenols. Gaocha, Kudingcha and tea phenols obviously can scavenge hydroxyl free radical. Within the range of density designed in this experimental the scaverging rate of coach phenols increases with the increase of the density, The conversion coefficient of the two was 0.9942, when reaching 0.7 mg/ml in density, its scavenging rate was about 100%; Within the range from 0 to 0.2mg/ml in density, the higher was Kudingcha phenols， density, the higher was its scavenging rate. When reaching 0.2 <WP=8>mg/ml in density, its maxium scavenging rate was about 35.05% the conversion coefficient of the two was 0.9985. From 0.2mg/ml or more in density, with the increase of the density, the scavenging rate of Kudingcha phenols decline. Within the range of density from 0 to 0.4mg/ml in density, the higher was tea polyphenols， density, the higher was its scavenging rate. The conversion coefficient of the two was 0.9953, when reaching 0.5 mg/ml in density, its max scavenging rate was about 89.6%. From 0.5mg/ml or more in density, was with the increase of the density, the scavenging rate of tea polyphenols decline.更多还原