【作者】 谭小兵；

【导师】 吴恩格；

【作者基本信息】 昆明医学院 ， 口腔临床医学， 2004， 硕士

【摘要】 目的：通过研究ZrO₂-CBC对体外培养SD胎鼠成骨细胞生长的影响，初步探讨ZrO₂-CBC对成骨细胞的毒性作用。 材料与方法：（1）以SD胎鼠颅盖骨组织为材料，体外培养原代成骨细胞，并对细胞进行鉴定、纯化、传代、扩增；（2）将ZrO₂-CBC和纯Ti制成厚度1.0mm、直径15.0mm的圆形薄片，清洁、消毒备用；（3）ZrO₂-CBC为实验组，纯Ti为对照组，将两组材料与成骨细胞在体外复合培养15d，分别在1d、5d、10d和15d评价两组材料对成骨细胞的影响。 结果：（1）培养出的细胞经形态学观察、ALP定性定量分析及OC检测，确定为高纯度、高活性的成骨细胞；（2）与纯Ti相比，实验早期ZrO₂-CBC对成骨细胞增殖分化无抑制作用（P＞0.05），后期轻度抑制成骨细胞的增殖，并抑制成骨细胞ALP的分泌（P＜0.05）；（3）电镜结果显示复合培养1d，成骨细胞就粘附在ZrO₂-CBC表面并开始生长。随着培养时间的延长，细胞数量不断增多，并有细胞突起伸入到ZrO₂-CBC内部，起到固定作用。 结论：本实验体外证明ZrO₂-CBC对SD胎鼠颅盖骨源性成骨细胞无明显毒性作用，是一种有前途的牙科种植体材料。更多还原

【Abstract】 Objective: To evaluate the cell compatibility of ZrO2-CBC by studying the influence of ZrO2-CBC on the growth of osteoblasts derived from SD fetal rats in vitro.Materials and Methods: Primary osteoblasts were cultured from SD fetal rats’ calvaria and then were identified, purified and passaged. ZrO2-CBC (as experiment group) and pure titanium (as control group) were prepared to be round thin slice (thickness 1.0mm, diameter 15.0mm) and then cleaned and sterilized for spare use. After that, both materials and osteoblasts were co-cultured in vitro for 15 days to evaluate the effect of the materials on cells in Id, 5d, 10d and 15d, respectively. Results: Cells derived from SD fetal rats’ calvaria were determined to be osteoblasts with higher purity and activity. It was found that ZrO2-CBC did not prevent the growth of osteoblasts at the earlyphase (P>0.05 ) , but it restrained the cells’ proliferation and ALP secretions atthe later phase (P<0.05) , compared with control group. Osteoblasts hadadhered to the surface of the material and begun to grow since the 1st day ofco-culture. The cells on the surface continued to increase and stretched into thedeep part of ZrO2-CBC.Conclusion: It was proved in vitro that ZrO2-CBC did no visible harm toosteoblasts derived from SD fetal rats’ calvaria. It is a promising dental implantmaterial.更多还原