【作者】 韩媛媛；

【导师】 阎正；

【作者基本信息】 河北大学 ， 分析化学， 2004， 硕士

【摘要】 本文共分三章。 第一章：文献综述，概括了中药指纹图谱的定义和属性，总结了中药指纹图谱研究的意义和作用，详细介绍了这一领域国内外研究现状，探讨了研究中药指纹图谱的技术问题。引用文献101篇。 第二章：本研究以东乐膏为载体，初步研究了中药橡胶膏剂指纹图谱的分析方法。采用GC、HPLC相结合的方式对其中不同性质的成分进行研究，可以较为全面地反映东乐膏中的化学组成。 利用CGC法对中药橡胶膏剂东乐膏中挥发性成分进行了分析，建立了东乐膏GC指纹图谱，确定8个色谱峰作为特征指纹峰，并对东乐膏中的主要挥发性成分冰片的含量进行了快速分离测定，分析周期为6min，线性范围为40～400mg／L，线性相关系数为0.9994。利用HPLC法对中药橡胶膏剂东乐膏进行了分离、分析，建立HPLC指纹图谱。色谱条件：Hypersil ODS2色谱柱(4.6×250 mm，5μm)，甲醇-1％醋酸(体积百分比浓度)为流动相，流速1.2 mL／min，检测波长254nm。该方法简便、准确、重现性好，符合相关技术要求。 第三章：应用CGC法对白芍、白芷及其所种植的土壤中有机氯农药残留的相关性进行了初步研究。样品以石油醚+丙酮(3：1)在索氏提取器中提取，提取液以浓硫酸净化。样品采用DB-5弹性石英毛细管柱分离，ECD检测。该方法简便、稳定、实用，应用此法考察了土壤中农药残留量对相关种植中草药的影响。更多还原

【Abstract】 This paper consists of three chapters.In CHAPTER ONE, literatures and reviews which contains definitions and attributions of fingerprint of Chinese medicine and the importance and effects of researching are summarized. Research progress in China and abroad are introduced in detailed. The technological problems of research of fingerprint of Chinese medicine are discussed. 101 pieces of literatures are cited.In CHAPTER TWO, Donglegao is the carrier of this study. Analytical method of the fingerprint of the traditional Chinese rubber electuary medicine is studied primarily. Integration method of GC and HPLC is employed in the study of different character contents and chemical components in Donglegao can be reflected overall.The fingerprint of Donglegao which is a traditional Chinese rubber electuary medicine has been studied and provides the quality information by capillary gas chromatography. The samples were pretreated by means of ultrasonic wave extraction with alcohol. The capillary column was DB-WAX ( 30m?.25mm?.25 祄, J&W Scientific). The content of borneolum in Donglegao was quickly determined. The analytical period was only 6min. The linear ranges were at least within 40-400mg/L ( R= 0.9994, n = 5). On the other hand, the fingerprint of Donglegao has been studied and provides the quality information by HPLC. Hypersil ODS2 column was used with mixtures of methanol and 1% acetic acid solution as mobile phase. The wavelength of UVD was 254 nm. The flow rate of mobile phase was 1.2 ml/min. The method is simple, accurate and reproducible. It may be practical value for the quality control of Donglegao.In CHAPTER THREE, A CGC method was introduced for the determination of organchlorine pesticide residues in Paeoniflorin, Radix Angelicae dahurica and the soil. The organochlorine pesticide residues were extracted with mixed solvents (Petroleumether+Acetone), cleaned up by concentrated sulfuric acid, separated by capillary column DB-5 (30m?.25mm?0.2 ?m ) and detected by ECD. The method was simple and reliable. The relativity of organchlorine pesticide residues between soil and Paeoniflorin was studied.更多还原