【作者】 潘亚妮；

【导师】 黄岳元；

【作者基本信息】 西北大学 ， 生物化工， 2004， 硕士

【摘要】 植物病害生物防治一直是农业研究的热点。低聚异麦芽糖是无毒、无任何副作用，不被微生物分解利用，易被动植物吸收和运输，可发酵规模化生产，是成本低廉的优良载体，故本论文以低聚异麦芽糖为原料，在研究其抑菌作用及成膜作用的基础上，对其进行化学修饰，在其自由碳上引入“-COO^-1”。经正交试验筛选得最优改性条件为：氯乙酸：低聚糖=4∶1，NaOH含量为40％，反应温度25℃，反应时间6h，含水量50％。研究了改性低聚异麦芽糖的各生理活性：吸湿率比低聚异麦芽糖提高93％，保湿率比低聚异麦芽糖提高160％；对柑桔青霉菌、西瓜枯萎、大豆炭疽菌、粉红聚端孢、茄褐斑菌、番茄叶芽枝霉菌、立枯丝核菌等7种病原真菌有明显抑菌作用，抑菌圈直径约1.5—2.7cm。10％的改性低聚糖溶液成膜后可完全抑制上述7种病原真菌的生长，而对灰霉菌抑制率大于50％；取代度为0.79的改性低聚异麦芽糖对氧自由基的清除率达32.61％，对氢自由基清除率达39.63％；应用于冷库猕猴桃灰霉病防治，能有效抑制灰霉病，提高猕猴桃硬度，延长保鲜时间，防治效果可达75.5％。更多还原

【Abstract】 The agriculture study has been focused on biocontrol of plant disease for a long time. Isomaltooligosaccharides(shorten for IMO) is a wonderful carrier. It is innocuity and no side-effect, at the same time, it can not be degraded by microorganism, further more, the cost of scale-up fermentation is quit low. Considering all of these, we choose IMO as material. We modified IMO after studying its inhibition and properties for membrane. The result of experimentation is as follows: ClCH2COOH: IMO =4:1,the content of NaOH is 40%, the reaction temperature is 25C,the reaction time is 6h and the content of water is 50%. Later on , we studied the physiological activities of this modified IMO: The moisture-absorption and moisture-retention are increased 93%and 160% respectively; The inhibition to 7 fungi is much in evidence. These fungi can not grow on the membrane of modified IMO at 10%concentrtion, but to Botrytic cinerea , the inhibition effect is higher than 50%;The modified IMO with 0. 79 substitute degree can scavenge oxygen free radical 32. 61%and hydroxyl free radical 39. 63%;Applying to Chinese goosebeery biocontrol, it can inhibit Botrytic disease, improve the fruit’ s hardness and prolong the green time. The biocontrol effect can be 75. 5%.更多还原