更年归肾丸对雌性去势大鼠生殖内分泌-免疫及自由基代谢影响的实验研究

【作者】 丁媛元；

【导师】 吴克明；

【作者基本信息】 成都中医药大学 ， 中医妇科学， 2004， 硕士

【摘要】 目的 通过观察更年归肾丸对更年期大鼠的生殖内分泌、免疫功能、自由基代谢的影响，探讨该方药对更年期综合征症状改善的作用机理。 方法 将3～4月龄SD雌性大鼠（200±20g）分为假手术组和去势组，去势组留取空白组外，分别给予更年归肾丸中剂量、更年归肾丸高剂量，并与妇复春胶囊进行对照。灌药20天后，处死大鼠，取血测血清雌二醇（E₂）、促卵泡生成激素（FSH）、促黄体生成激素（LH）、白细胞介素2（IL-2）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-P_x）、丙二醛（MDA）水平及胸腺、脾脏的重量。 结果 更年归肾丸能够明显升高去势大鼠血清IL-2含量；提高血清SOD、GSH—Px、降低MDA水平；而并不改变去势鼠血清E₂水平，对下丘脑—腺垂体亦未产生明显的影响；对去势后大鼠的胸腺、脾脏重量无明显改变。 结论 综合上述结果：更年归肾丸的作用机理可能并非是直接补充外源性雌激素，而是通过改善免疫功能及自由基代谢来间接改善更年期妇女临床症状，达到治疗更年期综合征的目的。更多还原

【Abstract】 Objective: To study the acting mechanism on improving sympt oms of climacteric syndrome through observing the effects on re productive endocrine, immune function and free radical metaboli sm of climacteric rats of Guishen pill.Methods: Female SD rats without pregnancy of 3-4 month-old were divided into pseudo-operated group and castrated group. Ca strated group were given treatments of high-dose, middle-dose G uishen pill and Fufuchun capsule respectively besides some rats without medicine. After being given medicine for twenty days, the rats were killed , then serum E2,FSH, LH, IL-2, SOD, GSH-PX, MDA level and quantity of thymus and spleen were measured.Results: Guishen pill can obviously increase the content ofserum IL-2 of castrated rats. It also can increase serum SOD and GSH-PX and decrease MDA level without changing serum E2 leve1 of castrated rats. It has no significant effects on hypothalamus-adenohypophys is.Conclusion: To sum up, the acting mechanism of Guishen pillis probably not supplementing outside-body estrogen directly but indirectly improving immune function and free radical metabo1 ism to improve clinic symptoms of climacteric women to achievethe goal of curing climacteric syndrome.更多还原